ARG45626

anti-PAR-3 / PARD3 antibody

anti-PAR-3 / PARD3 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes PAR-3 / PARD3
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PAR-3 / PARD3
Antigen Species Human
Immunogen Recombinant protein containing to human PAR-3 / PARD3.
Conjugation Un-conjugated
Alternate Names PARD3; Par-3 Family Cell Polarity Regulator; PAR3; ASIP; PPP1R118; Bazooka; PARD3A; Baz; Atypical PKC Isotype-Specific Interacting Protein; Protein Phosphatase 1, Regulatory Subunit 118; Par-3 Family Cell Polarity Regulator Alpha; Partitioning Defective 3 Homolog; CTCL Tumor Antigen Se2-5; PAR3-Alpha; PARD-3; Par-3 (Partitioning Defective 3, C.Elegans) Homolog; Par-3 Partitioning Defective 3 Homolog (C. Elegans); Atypical PKC Isotype-Specific-Interacting Protein; Par-3 Partitioning Defective 3 Homolog; PAR3alpha; SE2-5L16; SE2-5LT1; SE2-5T2; PAR3A; PAR-3

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 151 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 56288 Human PARD3

Swiss-port # Q8TEW0 Human Partitioning defective 3 homolog

Gene Symbol PARD3
Gene Full Name Par-3 Family Cell Polarity Regulator
Background This gene encodes a member of the PARD protein family. PARD family members interact with other PARD family members and other proteins; they affect asymmetrical cell division and direct polarized cell growth. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011]
Function Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. [UniProt]
Cellular Localization Cell junction; Cell membrane; Cytoplasm; Cytoskeleton; Membrane; Tight junction. [UniProt]
Calculated MW 151 kDa
PTM Acetylation; Phosphoprotein. [UniProt]

Images (4) Click the Picture to Zoom In

  • ARG45626 anti-PAR-3 / PARD3 antibody IHC-P image

    Immunohistochemistry: Human appendiceal adenocarcinoma stained with ARG45626 anti-PAR-3 / PARD3 antibody at 2 μg/ml dilution.

  • ARG45626 anti-PAR-3 / PARD3 antibody ICC/IF image

    Immunofluorescence: U2OS stained with ARG45626 anti-PAR-3 / PARD3 antibody at 5 μg/ml dilution.

  • ARG45626 anti-PAR-3 / PARD3 antibody WB image

    Western blot: MCF-7 and HEK293 stained with ARG45626 anti-PAR-3 / PARD3 antibody at 0.5 μg/ml dilution.

  • ARG45626 anti-PAR-3 / PARD3 antibody FACS image

    Flow Cytometry: HepG2 stained with ARG45626 anti-PAR-3 / PARD3 antibody at 1 µg/10^6 cells dilution.