ARG10786

anti-PDE10A antibody

anti-PDE10A antibody for Confocal microscopy (Confocal),ELISA,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot,Dot blot and Human,Monkey,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PDE10A
Tested Reactivity Hu, Ms, Rat, Mk
Tested Application Confocal, Dot, ELISA, ICC/IF, IHC, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PDE10A
Antigen Species Human
Immunogen Synthetic multiple antigenic peptide around the aa. 250-325 of Human PDE10A.
Conjugation Un-conjugated
Full Name phosphodiesterase 10A
Alternate Names HSPDE10A; cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A; EC 3.1.4.17; EC 3.1.4.35

Application Instructions

Application Suggestion
Tested Application Dilution
Confocal1:100 - 1:150
Dot1:10000
ELISA1:10000
ICC/IF1:100 - 1:150
IHC1:100 - 1:150
IP1:200
WB1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 88 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 10846 Human PDE10A

GeneID: 23984 Mouse PDE10A

GeneID: 63885 Rat PDE10A

Gene Symbol PDE10A
Gene Full Name phosphodiesterase 10A
Background The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase family. It plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This protein can hydrolyze both cAMP and cGMP to the corresponding nucleoside 5' monophosphate, but has higher affinity for cAMP, and is more efficient with cAMP as substrate. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Dec 2011]
Function Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate. [UniProt]
PTM Isoform PDE10A2: Phosphorylated on Thr-16.

Images (3) Click the Picture to Zoom In

  • ARG10786 anti-PDE10A antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat testis tissue section. A) Negative control, B) Stained with ARG10786 anti-PDE10A antibody at 1:200 dilution.

  • ARG10786 anti-PDE10A antibody WB image

    Western blot: Rat samples: 1) frontal cortex, 2) parietal cortex, 3) striatum, 4) amygdala, 5) hypothalamus, 6) hippocampus, 7) olfactory bulb, 8) brain stem, 9) cerebellum, 10) spinal cord, and 11) whole brain lysates stained with ARG10786 anti-PDE10A antibody at 1:500 dilution.

  • ARG10786 anti-PDE10A antibody WB image

    Western blot: Rat striatum lysates stained with ARG10786 anti-PDE10A antibody at 1:500 dilution.