ARG10791

anti-PDE1A antibody

anti-PDE1A antibody for Confocal microscopy (Confocal),ELISA,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot,Dot blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PDE1A
Tested Reactivity Hu, Ms, Rat
Tested Application Confocal, Dot, ELISA, ICC/IF, IHC, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PDE1A
Antigen Species Mouse
Immunogen Synthetic peptide around aa. 1-50 from Mouse PDE1A protein. Common to all PDE1A variants.
Conjugation Un-conjugated
Full Name phosphodiesterase 1A, calmodulin-dependent
Alternate Names CAM-PDE-1A; Cam-PDE 1A; HCAM1; Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A; EC 3.1.4.17; HSPDE1A; hCam-1; HCAM-1; 61 kDa Cam-PDE

Application Instructions

Application Suggestion
Tested Application Dilution
Confocal1:200
Dot1:10000
ELISA1:10000
ICC/IF1:200
IHC1:200
IP1:200
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 61 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 18573 Mouse PDE1A

GeneID: 5136 Human PDE1A

Swiss-port # P54750 Human Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A

Swiss-port # Q61481 Mouse Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A

Gene Symbol Pde1a
Gene Full Name phosphodiesterase 1A, calmodulin-dependent
Background Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109]; Fidock et al., 2002 [PubMed 11747989]).[supplied by OMIM, Oct 2009]
Function Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a higher affinity for cGMP than for cAMP. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG10791 anti-PDE1A antibody WB image

    Western blot: Recombinant PDE1A protein stained with ARG10791 anti-PDE1A antibody at 1:500 dilution.