ARG10792

anti-PDE1B antibody

anti-PDE1B antibody for Confocal microscopy (Confocal),ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot,Dot blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PDE1B
Tested Reactivity Hu, Ms, Rat
Tested Application Confocal, Dot, ELISA, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PDE1B
Species Human
Immunogen Synthetic peptide from Human PDE1B protein.
Conjugation Un-conjugated
Full Name phosphodiesterase 1B, calmodulin-dependent
Alternate Names Cam-PDE 1B; EC 3.1.4.17; 63 kDa Cam-PDE; PDES1B; Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B; PDE1B1; HEL-S-79p

Application Instructions

Application Suggestion
Tested Application Dilution
Confocal1:50 - 1:200
Dot1:10000
ELISA1:10000
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
IP1:200
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 61 kDa. Multiple bands in the 65 - 72 kDa range all believed to be various PDE1B variants.

Properties

Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 18574 Mouse PDE1B

GeneID: 29691 Rat PDE1B

GeneID: 5153 Human PDE1B

Gene Symbol PDE1B
Gene Full Name phosphodiesterase 1B, calmodulin-dependent
Background The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
Function Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG10792 anti-PDE1B antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human ovarian medulla tissue stained with ARG10792 anti-PDE1B antibody at 1:200 dilution.

  • ARG10792 anti-PDE1B antibody WB image

    Western blot: Recombinant PDE1B protein stained with ARG10792 anti-PDE1B antibody at 1:500 dilution.