ARG10797

anti-PDE3B antibody

anti-PDE3B antibody for Confocal microscopy (Confocal),ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot,Dot blot and Chicken,Human,Monkey,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PDE3B
Tested Reactivity Hu, Ms, Rat, Chk, Mk
Tested Application Confocal, Dot, ELISA, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PDE3B
Species Human
Immunogen Synthetic peptide taken within aa. 1060-1110 from Human PDE3B.
Conjugation Un-conjugated
Full Name phosphodiesterase 3B, cGMP-inhibited
Alternate Names EC 3.1.4.17; HcGIP1; cGIPDE1; CGIPDE1; CGI-PDE B; Cyclic GMP-inhibited phosphodiesterase B; cGMP-inhibited 3',5'-cyclic phosphodiesterase B; CGIP1

Application Instructions

Application Suggestion
Tested Application Dilution
Confocal1:250
Dot1:10000
ELISA1:10000
ICC/IF1:250
IHC-P1:250
IP1:200
WB1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 124 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 18576 Mouse PDE3B

GeneID: 29516 Rat PDE3B

GeneID: 5140 Human PDE3B

Gene Symbol PDE3B
Gene Full Name phosphodiesterase 3B, cGMP-inhibited
Function Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis. [UniProt]

Images (4) Click the Picture to Zoom In

  • ARG10797 anti-PDE3B antibody ICC/IF image

    Immunofluorescence: COS1 cells stained with ARG10797 anti-PDE3B antibody at 1:100 dilution.

  • ARG10797 anti-PDE3B antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human ovarian medulla tissue stained with ARG10797 anti-PDE3B antibody at 1:200 dilution.

  • ARG10797 anti-PDE3B antibody WB image

    Western blot: Recombinant PDE3B protein stained with ARG10797 anti-PDE3B antibody at 1:500 dilution.

  • ARG10797 anti-PDE3B antibody WB image

    Western blot: Adipose and HUVEC cell lysates stained with ARG10797 anti-PDE3B antibody.