anti-PDE4 antibody

anti-PDE4 antibody for Confocal microscopy (Confocal),Dot blot,ELISA,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Chicken,Human,Monkey,Mouse,Rat


Product Description Rabbit Polyclonal antibody recognizes PDE4
Tested Reactivity Hu, Ms, Rat, Chk, Mk
Tested Application Confocal, Dot, ELISA, ICC/IF, IHC, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PDE4
Antigen Species Human
Immunogen Synthetic peptide with a sequence common to most PDE4 subtypes: PDE4A, PDE4D, PDE4B.
Conjugation Un-conjugated
Alternate Names DPDE2; cAMP-specific 3',5'-cyclic phosphodiesterase 4A; PDE4; PDE46; EC

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.62 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 18577 Mouse PDE4A

GeneID: 25638 Rat PDE4A

GeneID: 5141 Human PDE4A

Gene Symbol PDE4A
Gene Full Name phosphodiesterase 4A, cAMP-specific
Background The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
Function Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. [UniProt]
Calculated MW 98 kDa
PTM Phosphorylation by MAPKAPK2 its activation through PKA phosphorylation (By similarity). Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA.
Proteolytically cleaved by caspase-3.

Images (1) Click the Picture to Zoom In

  • ARG10799 anti-PDE4 antibody WB image

    Western blot: Rat cortical membranes stained with ARG10799 anti-PDE4 antibody at 1:500 dilution.