ARG10800

anti-PDE4A antibody

anti-PDE4A antibody for Confocal microscopy (Confocal),ELISA,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot,Dot blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PDE4A
Tested Reactivity Hu, Ms, Rat
Tested Application Confocal, Dot, ELISA, ICC/IF, IHC, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PDE4A
Antigen Species Human
Immunogen Synthetic cyclic peptide around the C-terminus of PDE4A. Common to all PDE4A proteins.
Conjugation Un-conjugated
Full Name phosphodiesterase 4A, cAMP-specific
Alternate Names DPDE2; cAMP-specific 3',5'-cyclic phosphodiesterase 4A; PDE4; PDE46; EC 3.1.4.53

Application Instructions

Application Suggestion
Tested Application Dilution
Confocal1:100 - 1:400
Dot1:10000
ELISA1:10000
ICC/IF1:100 - 1:400
IHC
IP1:250
WB1:1000 - 1:2500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 98 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 18577 Mouse PDE4A

GeneID: 25638 Rat PDE4A

GeneID: 5141 Human PDE4A

Gene Symbol PDE4A
Gene Full Name phosphodiesterase 4A, cAMP-specific
Background The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
Function Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. [UniProt]
PTM Phosphorylation by MAPKAPK2 its activation through PKA phosphorylation (By similarity). Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA.
Proteolytically cleaved by caspase-3.

Images (6) Click the Picture to Zoom In

  • ARG10800 anti-PDE4A antibody IHC image

    Immunohistochemistry: Thick section of Mouse nose tissue stained with ARG10800 anti-PDE4A antibody (red). Green: Olfactory sensory neurons. Blue: Nuclei.

  • ARG10800 anti-PDE4A antibody WB image

    Western blot: 1) Recombinant PDE4A8, 2) Recombinant PDE4A5 & PDE4A1, and 3) Rat testis lysates stained with ARG10800 anti-PDE4A antibody at 1:500 dilution.

  • ARG10800 anti-PDE4A antibody Confocal image

    Scanning laser confocal microscopy: Fixed section of Rat seminefrous tubule stained with ARG10800 anti-PDE4A antibody.

  • ARG10800 anti-PDE4A antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human ovarian medulla tissue stained with ARG10800 anti-PDE4A antibody.

  • ARG10800 anti-PDE4A antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat kidney tissue stained with ARG10800 anti-PDE4A antibody at 1:100 dilution.

  • ARG10800 anti-PDE4A antibody IHC-Fr image

    Immunohistochemistry: Frozen section of Rat seminiferous tubule stained with ARG10800 anti-PDE4A antibody.