ARG10801

anti-PDE4B antibody

anti-PDE4B antibody for Confocal microscopy (Confocal),ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot,Dot blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PDE4B
Tested Reactivity Hu, Ms, Rat
Tested Application Confocal, Dot, ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PDE4B
Species Human
Immunogen Synthetic peptide common to all PDE4B subtypes taken near the N-terminus region.
Conjugation Un-conjugated
Full Name phosphodiesterase 4B, cAMP-specific
Alternate Names cAMP-specific 3',5'-cyclic phosphodiesterase 4B; EC 3.1.4.53; PDEIVB; DPDE4; PDE32

Application Instructions

Application Suggestion
Tested Application Dilution
Confocal1:250
Dot1:10000
ELISA1:10000
ICC/IF1:250
IHC-FrAssay-dependent
IHC-P1:250
IP1:350
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 83 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 24626 Rat PDE4B

GeneID: 5142 Human PDE4B

Swiss-port # P14646 Rat cAMP-specific 3',5'-cyclic phosphodiesterase 4B

Swiss-port # Q07343 Human cAMP-specific 3',5'-cyclic phosphodiesterase 4B

Gene Symbol PDE4B
Gene Full Name phosphodiesterase 4B, cAMP-specific
Background This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. The encoded protein regulates the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
Function Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents. [UniProt]

Images (4) Click the Picture to Zoom In

  • ARG10801 anti-PDE4B antibody IHC-Fr image

    Immunohistochemistry: Frozen section of seminiferous tubule stained with ARG10801 anti-PDE4B antibody.

  • ARG10801 anti-PDE4B antibody WB image

    Western blot: 1) Recombinant PDE4B variants, 2) Recombinant PDE4B3, and 3) Rat testis stained with ARG10801 anti-PDE4B antibody at 1:500 dilution.

  • ARG10801 anti-PDE4B antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human ovarian medulla tissue stained with ARG10801 anti-PDE4B antibody at 1:300 dilution.

  • ARG10801 anti-PDE4B antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human neuroblastoma (SHSY5Y) tissue stained with ARG10801 anti-PDE4B antibody at 1:250 dilution.