ARG43381
anti-PPP2R1A antibody
anti-PPP2R1A antibody for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PPP2R1A |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PPP2R1A |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-270 of Human PPP2R1A (NP_055040.2). |
| Conjugation | Un-conjugated |
| Alternate Names | Medium tumor antigen-associated 61 kDa protein; PP2A subunit A isoform R1-alpha; PP2A-Aalpha; PR65A; PP2A subunit A isoform PR65-alpha; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; MRD36; PP2AAALPHA |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | MCF7 | ||||||
| Observed Size | ~ 63 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P30153 Human Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha Swiss-port # Q76MZ3 Mouse Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha |
|---|---|
| Gene Symbol | PPP2R1A |
| Gene Full Name | protein phosphatase 2, regulatory subunit A, alpha |
| Background | This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010] |
| Function | The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT (PubMed:15525651). Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis (PubMed:16580887). [UniProt] |
| Cellular Localization | Cytoplasm. Chromosome, centromere. Lateral cell membrane. Cell projection, dendrite. Note=Centromeric localization requires the presence of BUB1. [UniProt] |
| Calculated MW | 65 kDa |
Images (2) Click the Picture to Zoom In
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ARG43381 anti-PPP2R1A antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG43381 anti-PPP2R1A antibody.
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ARG43381 anti-PPP2R1A antibody WB image
Western blot: 25 µg of MCF7 cell lysate stained with ARG43381 anti-PPP2R1A antibody at 1:500 dilution.
