ARG67224

anti-Paxillin antibody

anti-Paxillin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Paxillin
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Paxillin
Conjugation Un-conjugated
Alternate Names PXN; Paxillin; Testicular Tissue Protein Li 134

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:200 - 1:500
IHC-P1:200 - 1:500
WB1:1000 - 1:3000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified
Buffer 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300 and 20% Glycerol.
Preservative 0.025% ProClin 300
Stabilizer 20% Glycerol
Concentration 1.01 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 19303 Mouse PXN

GeneID: 360820 Rat PXN

GeneID: 5829 Human PXN

Gene Symbol PXN
Gene Full Name Paxillin
Background This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]
Function Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion. [Uniprot]
Cellular Localization Cell junction; Cytoplasm; Cytoskeleton. [Uniprot]
Calculated MW 65 kDa
PTM Acetylation; Phosphoprotein. [Uniprot]

Images (3) Click the Picture to Zoom In

  • ARG67224 anti-Paxillin antibody ICC/IF image

    Immunofluorescence: A431 stained with ARG67224 anti-Paxillin antibody.

  • ARG67224 anti-Paxillin antibody WB image

    Western blot: HeLa and A431 stained with ARG67224 anti-Paxillin antibody at 1:2000 dilution.

  • ARG67224 anti-Paxillin antibody IHC-P image

    Immunohistochemistry: Mouse cervix stained with ARG67224 anti-Paxillin antibody at 1:300 dilution.