ARG22296

anti-RAB4 antibody

anti-RAB4 antibody for ICC/IF,Immunohistochemistry,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes RAB4
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC, WB
Specificity Detects ~ 26 kDa.
Host Rabbit
Clonality Polyclonal
Target Name RAB4
Antigen Species Human
Immunogen C-terminal peptide from Human RAB4
Conjugation Un-conjugated
Alternate Names Ras-related protein Rab-4A; HRES1; RAB4; HRES-1; HRES-1/RAB4

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:150
IHC1:100
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 19341 Mouse RAB4A

GeneID: 25532 Rat RAB4A

GeneID: 5867 Human RAB4A

Gene Symbol RAB4A
Gene Full Name RAB4A, member RAS oncogene family
Background This gene is a member of the largest group in the Ras superfamily of small GTPases, which regulate membrane trafficking. The encoded protein is associated with early endosomes and is involved in their sorting and recycling. The protein also plays a role in regulating the recycling of receptors from endosomes to the plasma membrane. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2012]
Function Protein transport. Probably involved in vesicular traffic (By similarity). [UniProt]
Cellular Localization Cytoplasm, Membrane
Calculated MW 24 kDa
PTM Phosphorylated by CDK1 kinase during mitosis.

Images (4) Click the Picture to Zoom In

  • ARG22296 anti-RAB4 antibody ICC/IF image

    Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22296 anti-RAB4 antibody (yellow) at 1:150 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.

  • ARG22296 anti-RAB4 antibody IHC image

    Immunohistochemistry: Bouin solution fixed Mouse backskin stained with ARG22296 anti-RAB4 antibody (green) at 1:100 dilution (1 hour).

  • ARG22296 anti-RAB4 antibody WB image

    Western blot: Human HeLa cell lysates stained with ARG22296 anti-RAB4 antibody at 1:1000 dilution.

  • ARG22296 anti-RAB4 antibody ICC/IF image

    Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22296 anti-RAB4 antibody (red) at 1:150 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.