ARG66476

anti-RIPK1 / RIP1 phospho (Ser166) antibody [YJY-1-5]

anti-RIPK1 / RIP1 phospho (Ser166) antibody [YJY-1-5] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Mouse,Rat

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Overview

Product Description Rabbit Monoclonal antibody [YJY-1-5] recognizes RIPK1 / RIP1 phospho (Ser166)
Tested Reactivity Ms, Rat
Tested Application ICC/IF, IHC-Fr, IHC-P, IP, WB
Host Rabbit
Clonality Monoclonal
Clone YJY-1-5
Isotype IgG
Target Name RIPK1 / RIP1
Antigen Species Mouse
Immunogen Phosphospecific peptide around Ser166 of Mouse RIPK1 / RIP1.
Conjugation Un-conjugated
Alternate Names Receptor-interacting protein 1; RIP-1; Receptor-interacting serine/threonine-protein kinase 1; RIP; Cell death protein RIP; RIP1; EC 2.7.11.1; Serine/threonine-protein kinase RIP

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:500
IHC-Fr1:100 - 1:500
IHC-P1:100 - 1:500
IP1:50 - 1:500
WB1:1000 - 1:2000
Application Note IHC-P: Antigen Retrieval: Citrate buffer (pH 6.0) at high pressure and temperature.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 19766 Mouse RIPK1

Swiss-port # Q60855 Mouse Receptor-interacting serine/threonine-protein kinase 1

Gene Symbol RIPK1
Gene Full Name receptor (TNFRSF)-interacting serine-threonine kinase 1
Background RIPK1 / RIP1 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth. [provided by RefSeq, Aug 2017]
Function RIPK1 / RIP1: Serine-threonine kinase which is a key regulator of both cell death and cell survival (PubMed:25459879). Exhibits kinase activity-dependent functions that trigger cell death and kinase-independent scaffold functions regulating inflammatory signaling and cell survival (PubMed:11101870, PubMed:25459879). Initiates ripoptocide which describes cell death that is dependent on RIPK1, be it apoptosis or necroptosis (PubMed:31457011). Upon binding of TNF to TNFR1, RIPK1 is recruited to the TNF-R1 signaling complex (TNF-RSC also known as complex I) where it acts as a scaffold protein promoting cell survival, in part, by activating the canonical NF-kB pathway. Specific conditions can however activate RIPK1, and its kinase activity then regulates assembly of two death-inducing complexes, namely complex IIa (RIPK1-FADD-CASP8) and the complex IIb (RIPK1-RIPK3-MLKL) and these complexes respectively drive apoptosis or necroptosis, a regulated form of necrosis (PubMed:19524513, PubMed:19524512, PubMed:29440439, PubMed:30988283). During embryonic development suppresses apoptosis and necroptosis and prevents the interaction of TRADD with FADD thereby limiting aberrant activation of CASP8. Phosphorylates DAB2IP at 'Ser-728' in a TNF- alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade (PubMed:17389591). Required for ZBP1-induced NF-kappaB activation and activation of NF-kappaB by DNA damage and IR. [UniProt]
Cellular Localization Cytoplasm. Cell membrane. [UniProt]
Highlight Related products:
RIPK1 antibodies; RIPK1 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
RIP1 activation and pathogenesis of NASH
Calculated MW 76 kDa
PTM Proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apoptotic signaling through the TRADD-FADD interaction.

RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. Phosphorylation of Ser-161 by RIPK3 is necessary for the formation of the necroptosis-inducing complex.

Ubiquitinated by 'Lys-11'-, 'Lys-48'-, 'Lys-63'- and linear-linked type ubiquitin. Polyubiquitination with 'Lys-63'-linked chains by TRAF2 induces association with the IKK complex. Deubiquitination of 'Lys-63'-linked chains and polyubiquitination with 'Lys-48'-linked chains by TNFAIP3 leads to RIPK1 proteasomal degradation and consequently down-regulates TNF-alpha-induced NFkappa-B signaling. 'Lys-48'-linked polyubiquitination by RFFL or RNF34 also promotes proteasomal degradation and negatively regulates TNF-alpha-induced NFkappa-B signaling. Linear polyubiquitinated; the head-to-tail polyubiquitination is mediated by the LUBAC complex. LPS-mediated activation of NF-kappa-B. Also ubiquitinated with 'Lys-11'-linked chains. Polyubiquitinated with 'Lys-48' and 'Lys-63'-linked chains by BIRC2/c-IAP1 and BIRC3/c-IAP2, leading to activation of NF-kappa-B. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG66476 anti-RIPK1 / RIP1 phospho (Ser166) antibody [YJY-1-5] WB image

    Western blot: MLE cells untreated control (C) or treated with 20 ng/ml of TNF alpha, 1 μM of BV-6 and 20 μM of Z-VAD-FMK for 8 hours (T). Cell lysates were stained with ARG66476 anti-RIPK1 / RIP1 phospho (Ser166) antibody [YJY-1-5].

  • ARG66476 anti-RIPK1 / RIP1 phospho (Ser166) antibody [YJY-1-5] IHC-P image

    Immunohistochemistry: Paraffin-embedded rat lung tissue from rat silicosis model. Antigen Retrieval: Citrate buffer (pH 6.0) at high pressure and temperature. The tissue section was stained with ARG66476 anti-RIPK1 / RIP1 phospho (Ser166) antibody [YJY-1-5] at 1:70 dilution. The picture on the right is negative control.

Customer's Feedback

nuts_pic      Excellent

Review for anti-RIPK1 / RIP1 phospho (Ser166) antibody [YJY-1-5]

Application:WB

Sample:MLE cells untreated control (C) or treated with 20 ng/ml of TNF alpha, 1 μM of BV-6 and 20 μM of Z-VAD-FMK for 8 hours (T)

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-RIPK1 / RIP1 phospho (Ser166) antibody [YJY-1-5]

Application:IHC

Sample:Rat lung from rat silicosis model

Sample Preparation Method:Paraffin

Antigen Retrieval Buffer:Citrate acid buffer

Primary Antibody Dilution Factor:1:75

nuts_pic      Excellent

Review for anti-RIPK1 / RIP1 phospho (Ser166) antibody [YJY-1-5]

Application:IHC

Sample:Rat lung from rat silicosis model

Sample Preparation Method:Paraffin

Antigen Retrieval Buffer:Citrate buffer (pH 6.0)

Primary Antibody Dilution Factor:1:70

Specific References

NEK1-mediated retromer trafficking promotes blood-brain barrier integrity by regulating glucose metabolism and RIPK1 activation.

WB / Mouse

Huibing Wang et al.
Nat Commun.,  (2021)

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RIP1 kinase activity promotes steatohepatitis through mediating cell death and inflammation in macrophages.

WB, IHC-P / Mouse

Liang Tao et al.
Cell Death Differ.,  (2021)

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