ARG10653

anti-SERCA2 ATPase antibody

anti-SERCA2 ATPase antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description

Rabbit Polyclonal antibody recognizes SERCA2 ATPase

Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SERCA2 ATPase
Antigen Species Human
Immunogen Synthetic peptide around the center region of Human SERCA2 ATPase. 100% homologous in Human, Mouse and Rat. (QRDACLNARCFARVE)
Conjugation Un-conjugated
Full Name ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Alternate Names ATP2B; Endoplasmic reticulum class 1/2 Ca; SERCA2; SR Ca; DD; Sarcoplasmic/endoplasmic reticulum calcium ATPase 2; EC 3.6.3.8; Calcium pump 2; DAR; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; 2+

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P0.5 - 1 µg/ml
WB0.5 - 1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 115 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.025% Sodium azide and 2.5% BSA.
Preservative 0.025% Sodium azide
Stabilizer 2.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 11938 Mouse ATP2A2

GeneID: 29693 Rat ATP2A2

GeneID: 488 Human ATP2A2

Gene Symbol ATP2A2
Gene Full Name ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Background This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2008]
Function This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle. [UniProt]
PTM Nitrated under oxidative stress. Nitration on the two tyrosine residues inhibits catalytic activity.

Images (2) Click the Picture to Zoom In

  • ARG10653 anti-SERCA2 ATPase antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast cancer tissue stained with ARG10653 anti-SERCA2 ATPase antibody.

  • ARG10653 anti-SERCA2 ATPase antibody WB image

    Western blot: 1) Rat skeletal muscle, 2) Rat kidney, 3) Human PANC, and 4) SMMC-7721 cell lysates stained with ARG10653 anti-SERCA2 ATPase antibody.