ARG10751

anti-TAF15 antibody [4D71]

anti-TAF15 antibody [4D71] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse

Overview

Product Description Mouse Monoclonal antibody [4D71] recognizes TAF15
Tested Reactivity Hu, Ms
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 4D71
Isotype IgG1
Target Name TAF15
Antigen Species Human
Immunogen Full length recombinant Human TAF15 expressed in and purified from E. coli.
Conjugation Un-conjugated
Full Name TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
Alternate Names RBP56; TATA-binding protein-associated factor 2N; RNA-binding protein 56; TAF2N; II; Npl3; TAF; TAFII68; 68 kDa TATA-binding protein-associated factor

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:500 - 1:1000
IHC-Fr1:500 - 1:1000
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 62 kDa

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 8148 Human TAF15

Swiss-port # Q92804 Human TATA-binding protein-associated factor 2N

Gene Symbol TAF15
Gene Full Name TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
Background This gene encodes a member of the TET family of RNA-binding proteins. The encoded protein plays a role in RNA polymerase II gene transcription as a component of a distinct subset of multi-subunit transcription initiation factor TFIID complexes. Translocations involving this gene play a role in acute leukemia and extraskeletal myxoid chondrosarcoma, and mutations in this gene may play a role in amyotrophic lateral sclerosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
Function RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II). [UniProt]
PTM Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity.
ADP-ribosylated during genotoxic stress.

Images (4) Click the Picture to Zoom In

  • ARG10751 anti-TAF15 antibody [4D71] ICC/IF image

    Immunocytochemistry: HeLa cell cultures stained with ARG10751 anti-TAF15 antibody [4D71] (green) and co-stained with chicken antibody to vimentin (red). ARG10751 anti-TAF15 antibody [4D71] reveals a granular nuclear localization typical of many RNA associated proteins. Blue is a DNA stain.

  • ARG10751 anti-TAF15 antibody [4D71] ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG10751 anti-TAF15 antibody [4D71] (red) at 1:500 dilution and costained with Rabbit pAb to GAPDH (green) at 1:1000 dilution. Hoechst (blue) for nuclear staining.

    Clone 4D71 reveals TAF15 protein expressed exclusively in the nuclei of the cells, while GAPDH antibody produces strong cytoplasmic staining.

  • ARG10751 anti-TAF15 antibody [4D71] WB image

    Western blot: 20 µg of 1) HeLa,  and 2) Hek293 cell  lysates stained with ARG10751 anti-TAF15 antibody [4D71] at 1: 1000 dilution.

  • ARG10751 anti-TAF15 antibody [4D71] WB image

    Western blot: NIH/3T3 (cytosol), NIH/3T3 (nuclear), HeLa (cytosol) and HeLa (nuclear). The blots were stained with ARG10751 anti-TAF15 antibody [4D71] (green) at 1:1000 dilution.