ARG21166

anti-TCR gamma + TCR delta antibody [TCR-1] (FITC)

anti-TCR gamma + TCR delta antibody [TCR-1] (FITC) for Depletion,Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Chicken,Snake

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [TCR-1] recognizes TCR gamma + TCR delta
Tested Reactivity Chk, Snk
Tested Application Depletion, FACS, IHC-Fr, IHC-P
Specificity The clone TCR-1 precipitates a heterodimer of 90 kDa (two bands of 50 kDa and 40 kDa upon reduction) on chicken peripheral blood T cells. Deglycosylation of the heterodimer yields two polypeptides of 35 kDa and 32 kDa.
Host Mouse
Clonality Monoclonal
Clone TCR-1
Isotype IgG1, kappa
Target Name TCR gamma + TCR delta
Antigen Species Chicken
Immunogen Outbred chicken thymocytes and Ig-negative blood lymphocytes
Conjugation FITC
Full Name T cell receptor gamma locus; T cell receptor delta locus
Alternate Names TCR gamma: TCRG
TCR delta: TCRD; TCRDV1

Application Instructions

Application Suggestion
Tested Application Dilution
DepletionAssay-dependent
FACS< 1 µg/10^6 cells
IHC-FrAssay-dependent
IHC-PAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS and Sodium azide
Preservative Sodium azide
Concentration 0.5 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol TRG; TRD
Gene Full Name T cell receptor gamma locus; T cell receptor delta locus
Background TCR gamma: T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor gamma locus. The gamma locus includes V (variable), J (joining), and C (constant) segments. During T cell development, the gamma chain is synthesized by a recombination event at the DNA level joining a V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random addition of nucleotides by terminal deoxynucleotidyltransferase. Several V segments of the gamma locus are known to be incapable of encoding a protein and are considered pseudogenes. Somatic rearrangement of the gamma locus has been observed in T cells derived from patients with T cell leukemia and ataxia telangiectasia. [provided by RefSeq, Jul 2008]

Images (1) Click the Picture to Zoom In

  • ARG21166 anti-TCR gamma + TCR delta antibody [TCR-1] (FITC) FACS image

    Flow Cytometry: Chicken peripheral blood lymphocytes stained with ARG21166 anti-TCR gamma + TCR delta antibody [TCR-1] (FITC) and ARG21153 anti-CD3 antibody [CT-3] (PE).