ARG10001

anti-TNF alpha antibody [CH8820]

anti-TNF alpha antibody [CH8820] for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description

Mouse Monoclonal antibody [CH8820] recognizes TNF alpha

Tested Reactivity Hu
Tested Application ELISA, IHC-P, Neut
Host Mouse
Clonality Monoclonal
Clone CH8820
Isotype IgG1, k
Target Name TNF alpha
Antigen Species Human
Immunogen Purified recombinant human TNF alpha.
Conjugation Un-conjugated
Alternate Names Tumor necrosis factor ligand superfamily member 2; DIF; Cachectin; ICD2; ICD1; N-terminal fragment; TNF-a; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; NTF

Application Instructions

Application Suggestion
Application Dilution
ELISAAssay-dependent
IHC-P10 μg/ml
Neut1 μg antibody for 2-5 ng of recombinant TNF-alpha
Application Note ELISA: This antibody can be used as a capture antibody in sandwich ELISA in combination with a tracer/detection antibody (Cat. ARG10194 anti-TNF alpha antibody [B1E4] (HRP)).
Neutralizing: This antibody can neutralize TNF-alpha activity in vitro. Suggest use 2-5 ng of recombinant TNF-alpha per μg of mAb.
IHC: The antibody can react with formalin-fixed paraffin-embedded tissues. It showed strong reaction with normal kidney, liver with cirrhosis, and normal lung samples, and positive reaction with lung cancer samples at 10μg/mL antibody concentration.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control IHC-P: normal kidney, liver with cirrhosis, and normal lung samples

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.0)
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 7124 Human TNF

Swiss-port # P01375 Human Tumor necrosis factor

Gene Symbol TNF
Gene Full Name tumor necrosis factor
Background This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208).
The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. [UniProt]
Resrarch Area Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
PTM The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

Clone References

Vitamin E and C supplementation does not ameliorate muscle dysfunction after anterior cruciate ligament surgery.

IHC-P / Human

Barker T et al.
Free Radic Biol Med.,  (2009)

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