ARG52468

anti-Vimentin antibody

anti-Vimentin antibody for ICC/IF,IHC-Frozen sections,Immunohistochemistry (PFA perfusion fixed frozen sections),Western blot and Human,Mouse,Rat

Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; CAF Marker antibody; EMT Study antibody; Mesenchymal Markers antibody; Fibroblast Marker antibody; Muller Cell Marker antibody; Sarcoma Marker antibody

Overview

Product Description Chicken Polyclonal antibody recognizes Vimentin
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-FoFr , IHC-Fr, WB
Host Chicken
Clonality Polyclonal
Isotype IgY
Target Name Vimentin
Antigen Species Human
Immunogen Recombinant human vimentin purified from E. coli
Conjugation Un-conjugated
Full Name vimentin
Alternate Names Vimentin; CTRCT30; HEL113

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:500 - 1:5000
IHC-FoFr 1:500 - 1:5000
IHC-Fr1:500 - 1:5000
WB1:1000 - 1:10000
Application Note Specific for the ~50kDa Vimentin protein.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 54 kDa

Properties

Form Liquid
Purification Total IgY fraction
Buffer Total IgY fraction in PBS and 10 mM Sodium azide
Preservative 10 mM Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 22352 Mouse VIM

GeneID: 7431 Human VIM

GeneID: 81818 Rat VIM

Gene Symbol VIM
Gene Full Name vimentin
Background Vimentin is a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30146 Neural Stem / Progenitor Cell Marker Antibody Duo (Nestin, Vimentin)
Related products:
Vimentin antibodies; Vimentin Duos / Panels; Anti-Chicken IgY secondary antibodies;
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Research Area Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; CAF Marker antibody; EMT Study antibody; Mesenchymal Markers antibody; Fibroblast Marker antibody; Muller Cell Marker antibody; Sarcoma Marker antibody
PTM Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

Images (5) Click the Picture to Zoom In

  • ARG52468 anti-Vimentin antibody ICC/IF image

    Immunofluorescence: Mixed neuron/glial cultures stained with anti-Vimentin (green) and rabbit anti-GFAP antibody (ARG52312) (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two proteins assemble to produce heteropolymer filaments.

  • ARG52468 anti-Vimentin antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG52468 anti-Vimentin antibody (green) at 1:10000 dilution, and costained with anti-Actin antibody (red) at 1:500 dilution. DAPI (blue) for nuclear staining.

    The Vimentin antibody stains the intermediate filament network while the Actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.

  • ARG52468 anti-Vimentin antibody WB image

    Western Blot: NIH 3T3 cells showing specific immunolabeling of the ~50k Vimentin protein stained with ARG52468 Vimentin antibody.

  • ARG52468 anti-Vimentin antibody WB image

    Western blot: Rat brain, HeLa, SH-SY5Y, HEK293 and NIH/3T3 cell lysates stained with ARG52468 anti-Vimentin antibody (red) at 1:5000 dilution.

    The blot was simultaneously stained with ARG10720 anti-MAP2cd antibody [2C4] (green) at 1:5000 dilution, revealing multiple bands around 280 kDa that correspond to full length MAP2a/b isotypes while the ~ 70 kDa bands are MAP2c/d isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.

  • ARG52468 anti-Vimentin antibody ICC/IF image

    Immunofluorescence: Neuromics hN2 cells (derived from Human embryonic cell line WA09) were fixed and stained with ARG52468 anti-Vimentin antibody (red). Blue staining is nuclear DNA.