ARG55540

anti-Vimentin antibody

anti-Vimentin antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Monkey

Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
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5
anti-Vimentin antibody

Mouse Monoclonal antibody recognizes Vimentin

ARG55540 Arigo biolaboratories
4.4 out of 5 based on 5
anti-Vimentin antibody is rated 4.4 out of 5 by 5.

anti-Vimentin antibody

Overview

Product Description

Mouse Monoclonal antibody recognizes Vimentin

Tested Reactivity Hu, Mk
Tested Application ICC/IF, IP, WB
Specificity This antibody detects endogenous levels of Vimentin and does not cross-react with related proteins.
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Target Name Vimentin
Antigen Species Human
Immunogen Purified recombinant fragment of Human Vimentin protein (NP_003371.2).
Conjugation Un-conjugated
Full Name vimentin
Alternate Names Vimentin; CTRCT30; HEL113

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:800
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 54 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.4), 0.03% Proclin 300 and 50% Glycerol
Preservative 0.03% Proclin 300
Stabilizer 50% Glycerol
Concentration 1.64 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 7431 Human VIM

Swiss-port # P08670 Human Vimentin

Gene Symbol VIM
Gene Full Name vimentin
Background Vimentin is a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]
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Research Area Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
PTM Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

Images (6) Click the Picture to Zoom In

  • ARG55540 anti-Vimentin antibody ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG55540 anti-Vimentin antibody (green) at 1:800 dilution.

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG55540 anti-Vimentin antibody WB image

    Western blot: 20 µg of Colo205, HCT116, HT29, SW480 and SW620 cell lysates stained with ARG55540 anti-Vimentin antibody at 1:2000 dilution.

  • ARG55540 anti-Vimentin antibody IP image

    Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG55540 anti-Vimentin antibody.

  • ARG55540 anti-Vimentin antibody WB image

    Western blot: 20 µg of H1299 and A549 cell lysates stained with ARG55540 anti-Vimentin antibody at 1:1000 dilution.

  • ARG55540 anti-Vimentin antibody ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG55540 anti-Vimentin antibody (green) at 1:800 dilution.

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG55540 anti-Vimentin antibody WB image

    Western blot: 20 µg of 293T cell lysate stained with ARG55540 anti-Vimentin antibody at 1:1000 dilution.

Customer's Feedback

nuts_pic      Excellent

Review for anti-Vimentin antibody

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:800

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:FITC

nuts_pic      Good

Review for anti-Vimentin antibody

Application:WB

Sample:Colo205, HCT116, HT29, SW480 and SW620

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

Review for anti-Vimentin antibody

Application:WB

Sample:H1299 and A549

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-Vimentin antibody

Application:WB

Sample:293T

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

Review for anti-Vimentin antibody

Application:WB

Sample:A: MCF-7 (breast cancer) (negative control), B: MDA-MB-231 (meta breast cancer)

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC