anti-Vimentin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
|Product Description||Rabbit Polyclonal antibody recognizes Vimentin|
|Tested Reactivity||Hu, Ms, Rat|
|Tested Application||ICC/IF, IHC-P, WB|
|Immunogen||Recombinant protein of Human Vimentin.|
|Alternate Names||Vimentin; CTRCT30; HEL113|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Buffer||PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||vimentin|
|Background||This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]|
|Function||Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]
|PTM||Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. [UniProt]
Images (3) Click the Picture to Zoom In
ARG57642 anti-Vimentin antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG57642 anti-Vimentin antibody at 1:100 dilution.
ARG57642 anti-Vimentin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human stomach stained with ARG57642 anti-Vimentin antibody at 1:150 dilution.
ARG57642 anti-Vimentin antibody WB image
Western blot: 25 µg of Jurkat cell lysate stained with ARG57642 anti-Vimentin antibody at 1:1000 dilution.