ARG56085

anti-Vimentin antibody [LN-6]

anti-Vimentin antibody [LN-6] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Cat,Cow,Pig,Rat,Rabbit,Mouse,Sheep

Overview

Product Description Mouse Monoclonal antibody [LN-6] recognizes Vimentin
Tested Reactivity Hu, Ms, Rat, Cat, Cow, Pig, Rb, Sheep
Tested Application FACS, ICC/IF, IHC-P
Host Mouse
Clonality Monoclonal
Clone LN-6
Isotype IgM, kappa
Target Name Vimentin
Antigen Species Human
Immunogen A Human thymic nuclear extract.
Conjugation Un-conjugated
Full Name vimentin
Alternate Names Vimentin; CTRCT30; HEL113

Application Instructions

Application Suggestion
Tested Application Dilution
FACS0.5 - 1 µg/10^6 cells in 0.1ml
ICC/IF0.5 - 1 µg/ml
IHC-P1 - 2 µg/ml
Application Note Antigen retrieval for IHC-P: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification PEG precipitation
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 100522394 Pig VIM

GeneID: 22352 Mouse VIM

GeneID: 407747 Pig VIM

Gene Symbol VIM
Gene Full Name vimentin
Background This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]
Cellular Localization Cytoplasmic
PTM Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

Images (1) Click the Picture to Zoom In

  • ARG56085 anti-Vimentin antibody [LN-6] IHC-P image

    Immunohistochemistry: Formalin-fixed, paraffin-embedded Human melanoma stained with ARG56085 anti-Vimentin antibody [LN-6].