ARG56085

anti-Vimentin antibody [LN-6]

anti-Vimentin antibody [LN-6] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Cat,Cow,Pig,Rat,Rabbit,Mouse,Sheep

Overview

Product Description Mouse Monoclonal antibody [LN-6] recognizes Vimentin
Tested Reactivity Hu, Ms, Rat, Cat, Cow, Pig, Rb, Sheep
Tested Application FACS, ICC/IF, IHC-P
Host Mouse
Clonality Monoclonal
Clone LN-6
Isotype IgM, kappa
Target Name Vimentin
Antigen Species Human
Immunogen A Human thymic nuclear extract.
Conjugation Un-conjugated
Full Name vimentin
Alternate Names Vimentin; CTRCT30; HEL113

Application Instructions

Application Suggestion
Tested Application Dilution
FACS0.5 - 1 µg/10^6 cells in 0.1ml
ICC/IF0.5 - 1 µg/ml
IHC-P1 - 2 µg/ml
Application Note Antigen retrieval for IHC-P: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 54 kDa

Properties

Form Liquid
Purification PEG precipitation
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 100522394 Pig VIM

GeneID: 22352 Mouse VIM

GeneID: 407747 Pig VIM

Gene Symbol VIM
Gene Full Name vimentin
Background Vimentin is a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]
Cellular Localization Cytoplasmic
PTM Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

Images (1) Click the Picture to Zoom In

  • ARG56085 anti-Vimentin antibody [LN-6] IHC-P image

    Immunohistochemistry: Formalin-fixed, paraffin-embedded Human melanoma stained with ARG56085 anti-Vimentin antibody [LN-6].