ARG66302

anti-Vimentin antibody [SQab1859]

anti-Vimentin antibody [SQab1859] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Chicken,Dog,Goat,Hamster,Human,Monkey,Mouse,Pig,Rat,Xenopus,Zebrafish

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anti-Vimentin antibody [SQab1859] Mouse Monoclonal antibody [SQab1859] recognizes Vimentin ARG66302 Arigo biolaboratories
5.0 out of 5 based on 4
anti-Vimentin antibody [SQab1859] is rated 5.0 out of 5 by 4.

anti-Vimentin antibody [SQab1859]

Overview

Product Description Mouse Monoclonal antibody [SQab1859] recognizes Vimentin
Tested Reactivity Hu, Ms, Rat, Chk, Dog, Goat, Hm, Mk, Pig, Xenopus, Zfsh
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, WB
Specificity This antibody recognizes exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
Host Mouse
Clonality Monoclonal
Clone SQab1859
Isotype IgG1
Target Name Vimentin
Antigen Species Bovine
Immunogen A cytoskeletal Vimentin extract of bovine lens. 
Conjugation Un-conjugated
Full Name vimentin
Alternate Names Vimentin; CTRCT30; HEL113

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:100 - 1:200
ICC/IF1:50 - 1:250
IHC-Fr1:50 - 1:250
IHC-P1:50 - 1:250
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 54 kDa
Observed Size ~57 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 100522394 Pig VIM

GeneID: 22352 Mouse VIM

GeneID: 407747 Pig VIM

Gene Symbol VIM
Gene Full Name vimentin
Background This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]
Highlight Related products:
Vimentin antibodies; Vimentin Duos / Panels; Anti-Mouse IgG secondary antibodies;
PTM Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

Images (6) Click the Picture to Zoom In

  • ARG66302 anti-Vimentin antibody [SQab1859] WB image

    Western blot: 20 µg of 22RV1 and DU145 cell lysates stained with ARG66302 anti-Vimentin antibody [SQab1859] at 1:2000 dilution and ARG65680 anti-GAPDH antibody at 1:10000 dilution.

  • ARG66302 anti-Vimentin antibody [SQab1859] WB image

    Western blot: 20 µg of COLO205 and SW620 cell lysates stained with ARG66302 anti-Vimentin antibody [SQab1859] at 1:2000 dilution and ARG65680 anti-GAPDH antibody at 1:10000 dilution.

  • ARG66302 anti-Vimentin antibody [SQab1859] IHC-Fr image

    Immunohistochemistry: Frozen section of swine colon stained with ARG66302 anti-Vimentin antibody [SQab1859] (green) at 1:200 dilution. DAPI (blue) staining for cell nuclei.

  • ARG66302 anti-Vimentin antibody [SQab1859] WB image

    Western blot: 20 µg of 293T cell lysate stained with ARG66302 anti-Vimentin antibody [SQab1859] at 1:1000 dilution.

  • ARG66302 anti-Vimentin antibody [SQab1859] WB image

    Western blot: 20 µg of HeLa and 293T cell lysates stained with ARG66302 anti-Vimentin antibody [SQab1859] at 1:1000 dilution.

  • ARG66302 anti-Vimentin antibody [SQab1859] IHC-P image

    Immunohistochemistry: paraffin section of Human colon stained with ARG66302 anti-Vimentin antibody [SQab1859].

Customer's Feedback

nuts_pic      Excellent

Review for anti-Vimentin antibody [SQab1859]

Application:WB

Sample:COLO205 and SW620

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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nuts_pic      Excellent

Review for anti-Vimentin antibody [SQab1859]

Application:WB

Sample:22RV1 and DU145

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

show all review

nuts_pic      Excellent

Review for anti-Vimentin antibody [SQab1859]

Application:WB

Sample:HeLa and 293T

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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nuts_pic      Excellent

Review for anti-Vimentin antibody [SQab1859]

Application:WB

Sample:293T

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

show all review