ARG55244
anti-ZO1 antibody
anti-ZO1 antibody for Western blot and Human
Controls and Markers antibody
1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ZO1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ZO1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 1328-1362 (C-terminus) of Human ZO1. |
| Conjugation | Un-conjugated |
| Alternate Names | Zonula occludens protein 1; Tight junction protein ZO-1; Tight junction protein 1; Zona occludens protein 1; ZO-1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | A431 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TJP1 |
| Gene Full Name | tight junction protein 1 |
| Background | This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| Function | The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. [UniProt] |
| Cellular Localization | Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, tight junction. Cell junction Cell junction, gap junction. Note=Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact. Detected at the leading edge of migrating and wounded cells |
| Research Area | Controls and Markers antibody |
| Calculated MW | 195 kDa |
| PTM | Phosphorylated. Dephosphorylated by PTPRJ. |
Images (1) Click the Picture to Zoom In
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ARG55244 anti-ZO1 antibody WB image
Western blot: 20 µg of A431 cell lysate stained with ARG55244 anti-ZO1 antibody at 1:1000 dilution.
Customer's Feedback
Average
Review for anti-ZO1 antibody
Application：WB
Sample：293T cell lysate
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
