ARG22215
anti-alpha A Crystallin antibody [1H3.B8]
anti-alpha A Crystallin antibody [1H3.B8] for ELISA,ICC/IF,Western blot and Human,Mouse,Rat,Bovine
Overview
| Product Description | Mouse Monoclonal antibody [1H3.B8] recognizes alpha A Crystallin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov |
| Tested Application | ELISA, ICC/IF, WB |
| Specificity | Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1H3.B8 |
| Isotype | IgG1 |
| Target Name | alpha A Crystallin |
| Antigen Species | Human |
| Immunogen | Native Alpha Crystallin |
| Conjugation | Un-conjugated |
| Alternate Names | CRYA1; HSPB4; CTRCT9 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.2), 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CRYAA |
| Gene Full Name | crystallin alpha A |
| Cellular Localization | Cytoplasm, Nucleus |
| Highlight | Related products: anti-alpha A Crystallin antibody [1H3.B8]; |
| Calculated MW | 20 kDa |
Images (2) Click the Picture to Zoom In
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ARG22215 anti-alpha A Crystallin antibody [1H3.B8] ICC/IF image
Immunofluorescence: Human Neuroblastoma cell line SK-N-BE. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: ARG22215 anti-alpha A Crystallin antibody [1H3.B8] at 1:100 for 60 min at RT. Secondary Antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ARG22215 anti-alpha A Crystallin antibody [1H3.B8]. (D) Composite.
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ARG22215 anti-alpha A Crystallin antibody [1H3.B8] WB image
Western blot: Bovine tissue lysate stained with ARG22215 anti-alpha A Crystallin antibody [1H3.B8] at 1:1000 dilution. This blot shows absolute specificity as Left: AlphaA Crystallin, Right: AlphaB Crystallin.
