ARG22215

anti-alpha A Crystallin antibody [1H3.B8]

anti-alpha A Crystallin antibody [1H3.B8] for Western blot,ELISA,ICC/IF and Human,Mouse,Rat,Bovine

Overview

Product Description Mouse Monoclonal antibody [1H3.B8] recognizes alpha A Crystallin
Tested Reactivity Hu, Ms, Rat, Bov
Tested Application ELISA, ICC/IF, WB
Specificity Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Host Mouse
Clonality Monoclonal
Clone 1H3.B8
Isotype IgG1
Target Name alpha A Crystallin
Antigen Species Human
Immunogen Native Alpha Crystallin
Conjugation Un-conjugated
Alternate Names CRYA1; HSPB4; CTRCT9

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IF1:100
WB1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.2), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12954 Mouse CRYAA

GeneID: 1409 Human CRYAA

GeneID: 24273 Rat CRYAA

Gene Symbol CRYAA
Gene Full Name crystallin alpha A
Cellular Localization Cytoplasm, Nucleus
Calculated MW 20 kDa

Images (2) Click the Picture to Zoom In

  • ARG22215 anti-alpha A Crystallin antibody [1H3.B8] ICC/IF image

    Immunofluorescence: Human Neuroblastoma cell line SK-N-BE. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: ARG22215 anti-alpha A Crystallin antibody [1H3.B8] at 1:100 for 60 min at RT. Secondary Antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ARG22215 anti-alpha A Crystallin antibody [1H3.B8]. (D) Composite.

  • ARG22215 anti-alpha A Crystallin antibody [1H3.B8] WB image

    Western blot: Bovine tissue lysate stained with ARG22215 anti-alpha A Crystallin antibody [1H3.B8] at 1:1000 dilution. This blot shows absolute specificity as Left: AlphaA Crystallin, Right: AlphaB Crystallin.