ARG10186

anti-alpha Fetoprotein antibody [AFP-Y1]

anti-alpha Fetoprotein antibody [AFP-Y1] for ELISA,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody

Overview

Product Description

Mouse Monoclonal antibody [AFP-Y1] recognizes Human AFP

Tested Reactivity Hu
Tested Application ELISA, WB
Specificity No detectable cross-reaction with human normal serum proteins.
Host Mouse
Clonality Monoclonal
Clone AFP-Y1
Isotype IgG2b
Target Name alpha Fetoprotein
Antigen Species Human
Immunogen Human AFP, which was isolated from amniotic fluid and purified by affinity chromatography.
Conjugation Un-conjugated
Full Name alpha-fetoprotein
Alternate Names Alpha-fetoprotein; HPAFP; FETA; Alpha-fetoglobulin; AFPD; Alpha-1-fetoprotein

Application Instructions

Application Note ELISA: This antibody can be used as a capture antibody in sandwich immunoassay for human AFP detection.

Western blot: The antibody identifies 68kD AFP on western blot.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.0)
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 174 Human AFP

Swiss-port # P02771 Human Alpha-fetoprotein

Gene Symbol AFP
Gene Full Name alpha-fetoprotein
Background Alpha fetoprotein (AFP) is a 70kD glycoprotein which is produced primarily by liver cells in foetuses. It exists in significant amount in foetal plasma and decreases rapidly after birth. Moderate amount of AFP exists in serum of pregnant women. Monitoring mother’s serum AFP level in second trimester pregnancy can help assessing birth defects such as Down syndrome, neural tube defect and anencephaly. Elevated AFP level in men, children and non-pregnant women is associated with neoplastic conditions in lever, testicle or ovary.
Function Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. [UniProt]
Highlight Related products:
alpha Fetoprotein antibodies; alpha Fetoprotein ELISA Kits; alpha Fetoprotein Duos / Panels; Anti-Mouse IgG secondary antibodies;
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Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody
PTM Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated.

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  • ARG10186 anti-alpha Fetoprotein antibody [AFP-Y1] WB image

    Western blot: 20 µl of HepG2 and MDA-MB-231 cell lysates stained with ARG10186 anti-alpha Fetoprotein antibody [AFP-Y1] at 1:500 dilution.