anti-alpha Fetoprotein antibody [AFP-Y2] (HRP)

anti-alpha Fetoprotein antibody [AFP-Y2] (HRP) for ELISA and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody


Product Description HRP-conjugated Mouse Monoclonal antibody [AFP-Y2] recognizes Human AFP
Tested Reactivity Hu
Tested Application ELISA
Host Mouse
Clonality Monoclonal
Clone AFP-Y2
Isotype IgG1
Target Name alpha Fetoprotein
Antigen Species Human
Immunogen Human AFP, which was isolated from amniotic fluid and purified by affinity chromatography.
Conjugation HRP
Alternate Names Alpha-fetoprotein; HPAFP; FETA; Alpha-fetoglobulin; AFPD; Alpha-1-fetoprotein

Application Instructions

Application Note ELISA: In combination with capture antibody mAb AFP-Y1 (Cat. No.: ARG10186), this HRP conjugated antibody can be used as tracer antibody in sandwich ELISA assay for human AFP detection.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.2) and 50% Glycerol
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 174 Human AFP

Swiss-port # P02771 Human Alpha-fetoprotein

Gene Symbol AFP
Gene Full Name alpha-fetoprotein
Background Alpha fetoprotein (AFP) is a 70KD glycoprotein which is produced primarily by liver cells in foetuses. It exists in significant amount in foetal plasma and decreases rapidly after birth. Moderate amount of AFP exists in serum of pregnant women. Monitoring mother’s serum AFP level in second trimester pregnancy can help assessing birth defects such as Down syndrome, neural tube defect and anencephaly. Elevated AFP level in men, children and non-pregnant women is associated with neoplastic conditions in lever, testicle or ovary.
Function Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. [UniProt]
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Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody
Calculated MW 69 kDa
PTM Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.