ARG52485

anti-alpha smooth muscle Actin antibody [1A4]

anti-alpha smooth muscle Actin antibody [1A4] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human,Mouse,Pig,Rabbit,Rat,Sheep

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody
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anti-alpha smooth muscle Actin antibody [1A4]

Mouse Monoclonal antibody [1A4] recognizes alpha smooth muscle Actin (SMA)

ARG52485 Arigo biolaboratories
4.5 out of 5 based on 2
anti-alpha smooth muscle Actin antibody [1A4] is rated 4.5 out of 5 by 2.

anti-alpha smooth muscle Actin antibody [1A4]

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Overview

Product Description

Mouse Monoclonal antibody [1A4] recognizes alpha smooth muscle Actin (SMA)

Tested Reactivity Hu, Ms, Rat, Pig, Rb, Sheep
Tested Application ELISA, ICC/IF, IHC-Fr, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 1A4
Isotype IgG1, k
Target Name alpha smooth muscle Actin
Antigen Species Human
Immunogen Synthetic peptide from N-terminus of human alpha smooth muscle isoform of actin.
Conjugation Un-conjugated
Alternate Names Cell growth-inhibiting gene 46 protein; MYMY5; ACTSA; Alpha-actin-2; Actin, aortic smooth muscle; AAT6

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-Dependent
ICC/IFAssay-Dependent
IHC-FrAssay-Dependent
IHC-P1:200
WB1:100 - 1:300
Application Note IHC-P:
Incubation Time: 30 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Leiomyoma, Small Intestine (Smooth Muscle)
Calculated MW 42 kDa

Properties

Form Liquid
Buffer Tissue culture supernatant in TBS (pH 7.5), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 100009271 Rabbit ACTA2

GeneID: 11475 Mouse ACTA2

GeneID: 59 Human ACTA2

Background This antibody stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained. It reacts with tumors arising from smooth muscles and myoepithelial cells. Actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric), and myocardium (alpha-myocardial) are not stained.
Cellular Localization Cytoplasm
Highlight Related products:
alpha smooth muscle Actin antibodies; alpha smooth muscle Actin Duos / Panels; Anti-Mouse IgG secondary antibodies;
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Research Area Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody
PTM Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).
Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).

Images (2) Click the Picture to Zoom In

  • ARG52485

    Western blot: 30 µg of MEF cell lysate stained with anti-alpha smooth muscle Actin antibody [1A4] at 1:200 dilution.

  • ARG52485 anti-Actin-Smooth Muscle antibody [1A4] IHC-P image

    Immunohistochemistry: Human Small Intestine stained with Actin-Smooth Muscle antibody [1A4] (ARG52485)

Customer's Feedback

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Review for anti-alpha smooth muscle Actin antibody [1A4]

Application:WB

Sample:Rat heart

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:300

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

show all review

nuts_pic      Excellent

Review for anti-alpha smooth muscle Actin antibody [1A4]

Application:WB

Sample:MEF cell lysate

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

show all review

Specific References

Establishing a nonlethal and efficient mouse model of male gonadotoxicity by intraperitoneal busulfan injection.

IHC-P / Mouse

Xie Yun et al.
Asian J Androl.,  (2019)

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Macrophage migration inhibitory factor is critical for dengue NS1-induced endothelial glycocalyx degradation and hyperpermeability

IHC-P / Mouse

Chen Hong-Ru et al.
PLoS Pathog.,  (2018)

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Preparation and renoprotective effects of carboxymethyl chitosan oligosaccharide on adriamycin nephropathy

IHC-P / Rat

Qiao Jing et al.
Carbohydrate Polymers.,  (2018)

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