ARG10760

anti-mCherry antibody

anti-mCherry antibody for ICC/IF,IHC-Frozen sections,Western blot and Other

Overview

Product Description

Rabbit Polyclonal Antibody to mCherry red fluorescent protein

Tested Reactivity Other
Tested Application ICC/IF, IHC-Fr, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name mCherry
Antigen Species Others
Immunogen Full length recombinant protein.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:500
IHC-Fr1:500
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (3) Click the Picture to Zoom In

  • ARG10760 anti-mCherry antibody ICC/IF image

    Immunocytochemistry: HEK293 cells transfected in the same way and viewed using a confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are red. Staining with ARG10760 anti-mCherry antibody is shown in Green. Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Interestingly, stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules to release the low molecular weight mCherry fluorochrome.

  • ARG10760 anti-mCherry antibody WB image

    Western blot: HEK293 cells transfected with pFin-EF1-mCherry vector ("+"), and HEK293 cells which were not transfected with this vector ("-"). The blots were stained with ARG10760 anti-mCherry antibody.

  • ARG10760 anti-mCherry antibody WB image

    Western blot: 1) HEK293 (non-transfected), 2) HEK293 transfected with mCherry-HA construct, 3) Recombinant mCherry protein, 4) Recombinant GFP protein and 5) HEK293 transfected with a full length GFP construct. The blots were stained with ARG10760 anti-mCherry antibody (green) at 1:1000 dilution. The same blot was simultaneously stained with ARG10757 anti-Hsp 60 antibody (red) at 1:5000 dilution.