ARG81351

HMGB1 ELISA Kit

HMGB1 ELISA Kit for ELISA and Human,Mouse,Rat

Overview

Product Description

ARG81351 HMGB1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human/Mouse/Rat HMGB1 in serum samples.

Tested Reactivity Hu, Ms, Rat
Tested Application ELISA
Target Name HMGB1
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm.

Sensitivity 0.4 ng/ml
Sample Type Serum
Standard Range 0.625 - 20 ng/ml
Sample Volume 50 µl
Full Name high mobility group box 1
Alternate Names HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3

Application Instructions

Assay Time ~ 2 hours

Properties

Form 96 well
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 15289 Mouse HMGB1

GeneID: 3146 Human HMGB1

Swiss-port # P09429 Human High mobility group protein B1

Swiss-port # P63158 Mouse High mobility group protein B1

Gene Symbol HMGB1
Gene Full Name high mobility group box 1
Background This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
Function DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells (By similarity). [UniProt]
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PTM Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion (PubMed:17114460).

Acetylated on multiple sites upon stimulation with LPS (PubMed:22801494). Acetylation on lysine residues in the nuclear localization signals (NLS 1 and NLS 2) leads to cytoplasmic localization and subsequent secretion (By similarity). Acetylation on Lys-3 results in preferential binding to DNA ends and impairs DNA bending activity (By similarity).

Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB1 (HMGB1C23hC45hC106h), 2- disulfide HMGB1 (HMGB1C23-C45C106h) and 3- sulfonyl HMGB1 (HMGB1C23soC45soC106so).

Poly-ADP-ribosylated by PARP1 when secreted following stimulation with LPS (By similarity).

In vitro cleavage by CASP1 is liberating a HMG box 1-containing peptide which may mediate immunogenic activity; the peptide antagonizes apoptosis-induced immune tolerance (PubMed:24474694). Can be proteolytically cleaved by a thrombin:thrombomodulin complex; reduces binding to heparin and proinflammatory activities (By similarity). [UniProt]

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  • ARG81351 HMGB1 ELISA Kit standard curve image

    ARG81351 HMGB1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81351 HMGB1 ELISA Kit User's manual download icon Download