ARG81185

Human HMGB1 ELISA Kit

Human HMGB1 ELISA Kit for ELISA and Human

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Component

Cat No Component Name Package Temp
ARG81185-001 antibody-coated microplate 12 X 8 strips 4°C
ARG81185-002 Standard (100 ng) 1 vial (lyophilized) 4°C, store at -20 °C after reconstitution
ARG81185-003 Standard reconstitution buffer 1.1 ml 4°C
ARG81185-004 HRP-antibody Conjugate (200X) 115 μl 4°C
ARG81185-005 Sample & Antibody diluent buffer 50 ml 4°C
ARG81185-006 10X Wash buffer 20 ml 4°C
ARG81185-007 TMB substrate 12 ml (ready-to-use) 4°C (Protect from light)
ARG81185-008 STOP solution 6 ml 4°C

Overview

Product Description

ARG81185 Human HMGB1 ELISA Kit is an Enzyme Immunoassay kit in simple steps with good precision for the quantification of Human HMGB1 in plasma and cell culture supernatant.

Tested Reactivity Hu
Predict Reactivity Ms, Rat
Tested Application ELISA
Specificity This Human HMGB1 ELISA kit detect natural and recombinant Human HMGB1 protein and the cross-reactivity with HMGB2 is lower than <2%.
Target Name HMGB1
Sensitivity 0.3 ng/ml
Sample Type Plasma, and cell culture supernatant
Standard Range 0.3125 - 20 ng/ml
Sample Volume 100 µl
Precision

< 5.2%

Full Name high mobility group box 1
Alternate Names HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3

Application Instructions

Assay Time Overnight

Properties

Form 96 well
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3146 Human HMGB1

Swiss-port # P09429 Human High mobility group protein B1

Gene Symbol HMGB1
Gene Full Name high mobility group box 1
Background This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
Function DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). [UniProt]
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PTM Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion (PubMed:17114460).
Acetylated on multiple sites upon stimulation with LPS (PubMed:22801494). Acetylation on lysine residues in the nuclear localization signals (NLS 1 and NLS 2) leads to cytoplasmic localization and subsequent secretion (By similarity). Acetylation on Lys-3 results in preferential binding to DNA ends and impairs DNA bending activity (By similarity).
Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB1 (HMGB1C23hC45hC106h), 2- disulfide HMGB1 (HMGB1C23-C45C106h) and 3- sulfonyl HMGB1 (HMGB1C23soC45soC106so).
Poly-ADP-ribosylated by PARP1 when secreted following stimulation with LPS (By similarity).
In vitro cleavage by CASP1 is liberating a HMG box 1-containing peptide which may mediate immunogenic activity; the peptide antagonizes apoptosis-induced immune tolerance (PubMed:24474694). Can be proteolytically cleaved by a thrombin:thrombomodulin complex. (By similarity).

Images (2) Click the Picture to Zoom In

  • ARG81185 Human HMGB1 ELISA Kit standard curve image

    ARG81185 Human HMGB1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

  • ARG81185 Human HMGB1 ELISA Kit specificity testing image

    Recombinant HMGB1 and recombinant HMGB2 were used for ARG81185 Human HMGB1 ELISA Kit specificity test. The data showed no significant cross-reactivity or interference with 25 ng/mL of recombinant human HMGB2 was observed.

Specific References

High-mobility group box 1 protein modulated Proliferation and Radioresistance in Esophageal Squamous Cell Carcinoma.

ELISA / Human

Di Xiaoke et al.
J Gastroenterol Hepatol.,  (2018)

publication_link

 

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Title Download Link
ARG81185 Human HMGB1 ELISA Kit User's manual download icon Download