Human HMGB1 ELISA Kit
Human HMGB1 ELISA Kit for ELISA and Human
|Cat No||Component Name||Package||Temp|
|ARG81185-001||antibody-coated microplate||12 X 8 strips||4°C|
|ARG81185-002||Standard (100 ng)||1 vial (lyophilized)||4°C, store at -20 °C after reconstitution|
|ARG81185-003||Standard reconstitution buffer||1.1 ml||4°C|
|ARG81185-004||HRP-antibody Conjugate (200X)||115 μl||4°C|
|ARG81185-005||Sample & Antibody diluent buffer||50 ml||4°C|
|ARG81185-006||10X Wash buffer||20 ml||4°C|
|ARG81185-007||TMB substrate||12 ml (ready-to-use)||4°C (Protect from light)|
|ARG81185-008||STOP solution||6 ml||4°C|
|Product Description||ARG81185 Human HMGB1 ELISA Kit is an Enzyme Immunoassay kit in simple steps with good precision for the quantification of Human HMGB1 in plasma and cell culture supernatants.|
|Predict Reactivity||Ms, Rat|
|Specificity||This Human HMGB1 ELISA kit detect natural and recombinant Human HMGB1 protein and the cross-reactivity with HMGB2 is lower than <2%.|
|Conjugation Note||Substrate: TMB and read at 450 nm.|
|Sample Type||Plasma and cell culture supernatants.|
|Standard Range||0.3125 - 20 ng/ml|
|Sample Volume||100 µl|
|Alternate Names||HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3|
|Storage Instruction||Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||high mobility group box 1|
|Background||HMGB1 is a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]|
|Function||HMGB1 is a DNA binding protein. It associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). [UniProt]|
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|PTM||Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion (PubMed:17114460).
Acetylated on multiple sites upon stimulation with LPS (PubMed:22801494). Acetylation on lysine residues in the nuclear localization signals (NLS 1 and NLS 2) leads to cytoplasmic localization and subsequent secretion (By similarity). Acetylation on Lys-3 results in preferential binding to DNA ends and impairs DNA bending activity (By similarity).
Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB1 (HMGB1C23hC45hC106h), 2- disulfide HMGB1 (HMGB1C23-C45C106h) and 3- sulfonyl HMGB1 (HMGB1C23soC45soC106so).
Poly-ADP-ribosylated by PARP1 when secreted following stimulation with LPS (By similarity).
In vitro cleavage by CASP1 is liberating a HMG box 1-containing peptide which may mediate immunogenic activity; the peptide antagonizes apoptosis-induced immune tolerance (PubMed:24474694). Can be proteolytically cleaved by a thrombin:thrombomodulin complex. (By similarity).
Images (2) Click the Picture to Zoom In
ARG81185 Human HMGB1 ELISA Kit standard curve image
ARG81185 Human HMGB1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
ARG81185 Human HMGB1 ELISA Kit specificity testing image
Recombinant HMGB1 and recombinant HMGB2 were used for ARG81185 Human HMGB1 ELISA Kit specificity test. The data showed no significant cross-reactivity or interference with recombinant human HMGB2.
|ARG81185 Human HMGB1 ELISA Kit User's manual||Download|