ARG80101

Human IL1 beta ELISA Kit

Human IL1 beta ELISA Kit for ELISA and Human

Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit

Component

Cat No Component Name Package Temp
ARG80101-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80101-002 Standard (Lyophilized) 3 X 0.8 ng/vial 4°C
ARG80101-003 Standard diluent buffer 20 ml 4°C
ARG80101-004 Antibody conjugate concentrate 400 µl 4°C
ARG80101-005 Antibody diluent buffer 16 ml 4°C
ARG80101-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80101-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80101-008 20X Wash buffer 50 ml 4°C
ARG80101-009 TMB substrate 12ml 4°C (Protect from light)
ARG80101-010 STOP solution 12ml 4°C
ARG80101-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80101 Human IL1 beta ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL1 beta in Serum, Plasma, Cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human IL-1 alpha;mouse IL-1 alpha, IL-1 beta;rat IL-1 alpha;porcine IL-1 alpha, IL-1 beta
Target Name IL1 beta
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 4 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 7.8 - 500 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Full Name interleukin 1, beta
Alternate Names Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IL1B
Gene Full Name interleukin 1, beta
Background The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
Function Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. [UniProt]
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Research Area Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit
PTM Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.

Images (1) Click the Picture to Zoom In

  • ARG80101 Human IL1 beta ELISA Kit standard curve image

    ARG80101 Human IL1 beta ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG80101 Human IL1 beta ELISA Kit User's manual download icon Download