ARG80196

Mouse IL1 beta ELISA Kit

Mouse IL1 beta ELISA Kit for ELISA and Mouse

Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit
publication_link Publications4

Component

Cat No Component Name Package Temp
ARG80196-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80196-002 Standard (Lyophilized) 3 X 0.8 ng/vial 4°C
ARG80196-003 Standard diluent buffer 20 ml 4°C
ARG80196-004 Antibody conjugate concentrate 400 µl 4°C
ARG80196-005 Antibody diluent buffer 16 ml 4°C
ARG80196-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80196-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80196-008 20X Wash buffer 50 ml 4°C
ARG80196-009 TMB substrate 12ml 4°C (Protect from light)
ARG80196-010 STOP solution 12ml 4°C
ARG80196-011 Plate sealer 6 strips Room temperature

Overview

Product Description

ARG80196 Mouse IL1 beta ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IL1 beta in Serum, Plasma, Cell culture supernatants.

Tested Reactivity Ms
Tested Application ELISA
Specificity No significant cross-reactivity or interference with mouse IL-1 alpha, IL-1ra, IL-1 RI/Fc Chimera, IL-1 RII/Fc Chimera
Target Name IL1 beta
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 8 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Full Name interleukin 1 beta
Alternate Names Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 16176 Mouse IL1B

Swiss-port # P10749 Mouse Interleukin-1 beta

Gene Symbol Il1b
Gene Full Name interleukin 1 beta
Background Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.
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Research Area Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit
PTM Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.

Images (1) Click the Picture to Zoom In

  • ARG80196 Mouse IL1 beta ELISA Kit standard curve image

    ARG80196 Mouse IL1 beta ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Specific References

Red Seaweed Eucheuma cottonii Ethanol Extract Regulates Inflammatory Mediators Production and Prevents Colonic Injury on Acute Colitis Disease in Mice

ELISA / Mouse

Sabri Sudirman et al.
Preprint.,  (2018)

publication_link

 

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Influences of Surface Coating of PLGA Nanoparticles on Immune Activation of Macrophages.

ELISA / Mouse

Chen Xinyi et al.
J. Mater. Chem. B,  (2018)

publication_link

 

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Influences of size and surface coating of gold nanoparticles on inflammatory activation of macrophages.

ELISA / Mouse

Chen Xinyi et al.
Colloids Surf B Biointerfaces.,  (2017)

publication_link

 

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Therapeutic effects of matrine derivate MASM in mice with collagen-induced arthritis and on fibroblast-like synoviocytes.

ELISA / Mouse

Yuming Zou et al.
Sci Rep. ,  (2017)

publication_link

 

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Title Download Link
ARG80196 Mouse IL1 beta ELISA Kit User's manual download icon Download