ARG81310

Mouse/Rat HMGB1 ELISA Kit

Mouse/Rat HMGB1 ELISA Kit for ELISA and Mouse,Rat

publication_link Publication8

Overview

Product Description ARG81310 Mouse/Rat HMGB1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse/Rat HMGB1 in plasma, cell culture supernatants and cell/tissue lysate samples.
Tested Reactivity Ms, Rat
Tested Application ELISA
Specificity Cross-react to mouse HMGB2 is less than 5%.
Target Name HMGB1
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm.

Sensitivity 0.3 ng/ml
Sample Type Plasma, cell culture supernatants and cell/tissue lysate samples.
Standard Range 0.3125 - 20 ng/ml
Sample Volume 100 µl
Precision

< 5.2%

Full Name high mobility group box 1
Alternate Names HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3

Application Instructions

Assay Time Overnight

Properties

Form 96 well
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 15289 Mouse HMGB1

Swiss-port # P63158 Mouse High mobility group protein B1

Gene Symbol Hmgb1
Gene Full Name high mobility group box 1
Background HMGB1 is a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
Function HMGB1 is a DNA binding protein. It associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). [UniProt]
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PTM Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion (PubMed:17114460).
Acetylated on multiple sites upon stimulation with LPS (PubMed:22801494). Acetylation on lysine residues in the nuclear localization signals (NLS 1 and NLS 2) leads to cytoplasmic localization and subsequent secretion (By similarity). Acetylation on Lys-3 results in preferential binding to DNA ends and impairs DNA bending activity (By similarity).
Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB1 (HMGB1C23hC45hC106h), 2- disulfide HMGB1 (HMGB1C23-C45C106h) and 3- sulfonyl HMGB1 (HMGB1C23soC45soC106so).
Poly-ADP-ribosylated by PARP1 when secreted following stimulation with LPS (By similarity).
In vitro cleavage by CASP1 is liberating a HMG box 1-containing peptide which may mediate immunogenic activity; the peptide antagonizes apoptosis-induced immune tolerance (PubMed:24474694). Can be proteolytically cleaved by a thrombin:thrombomodulin complex.

Images (1) Click the Picture to Zoom In

  • ARG81310 Mouse/Rat HMGB1 ELISA Kit standard curve image

    ARG81310 Mouse/Rat HMGB1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Specific References

Molecular hydrogen alleviates brain injury and cognitive impairment in a chronic sequelae model of murine polymicrobial sepsis.

ELISA / Mouse / Tissue lysate

Yi Jiang et al.
Exp Brain Res.,  (2020)

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Tumor-derived HMGB1 induces CD62L dim neutrophil polarization and promotes lung metastasis in triple-negative breast cancer.

ELISA / Mouse / Serum and culture medium

Zhen Wang et al.
Oncogenesis.,  (2020)

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Carrier-free nanoassembly of doxorubicin prodrug and siRNA for combinationally inducing immunogenic cell death and reversing immunosuppression.

ELISA / Mouse / Cell culture supernatant

Senbiao Chen et al.
Nano Today.,  (2020)

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Inhibition of HMGB1 Promotes Osseointegration under Hyperglycemic Condition through Improvement of BMSC Dysfunction.

ELISA / Rat

Liu Beilei et al.
Oxid Med Cell Longev.,  (2019)

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Syringeable immunotherapeutic nanogel reshapes tumor microenvironment and prevents tumor metastasis and recurrence.

ELISA / Mouse

Song Chanyoung et al.
Nat Commun.,  (2019)

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Targeting HMGB1 by ethyl pyruvate ameliorates systemic lupus erythematosus and reverses the senescent phenotype of bone marrow-mesenchymal stem cells.

ELISA / 

Ji Juan et al.
Aging.,  (2019)

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Irradiation-induced osteocyte damage promotes HMGB1-mediated osteoclastogenesis in vitro.

ELISA / Mouse

He Feilong et al.
J Cell Physiol.,  (2019)

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Carbon ion irradiation enhances the antitumor efficacy of dual immune checkpoint blockade therapy both for local and distant sites in murine osteosarcoma.

ELISA / Mouse

Takahashi Yutaka et al.
Oncotarget.,  (2019)

publication_link

 

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Title Download Link
ARG81310 Mouse/Rat HMGB1 ELISA Kit User's manual download icon Download