ARG30001

Tag Internal Control Antibody Duo (GFP, GAPDH)

Cancer antibody; Controls and Markers antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
publication_link Publication31

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG10112 anti-GAPDH antibody [6C5] Mouse mAb AGMK, Bb, Cat, Chk, Dog, Fsh, Hm, Hu, Mk, Ms, Pig, Rb, Rat, Xenopus laevis, Zfsh ELISA, ICC/IF, IHC-Fr, WB 50 μg
ARG62343 anti-GFP antibody [GF28R] Mouse mAb Other Dot, ELISA, ICC/IF, IP, WB 50 μg

Overview

Product Description

GFP has become one of the most important tools used in bioscience. When using GFP as a reporter for protein expressing study, arigo recommend to include GAPDH as a loading control to normalize the sample loading and the experimental errors to show the convincing result. GAPDH is often stably and constitutively expressed at high levels in most tissues and cells in cytoplasm. Therefore, GAPDH is a widely used loading control and cytoplasm marker for western blot (35-40kDa) and ICC/IF study. Usage note: S-nitrosylated GAPDH might translocate to nucleus especially for nitric oxide-related studies. In addition, the GAPDH expression level is up regulated under hypoxia condition. Therefore, GAPDH is not suitable for loading control and cytoplasm marker for oxygen-related and nitric oxide-related studies. GFP, green fluorescent protein, is a protein from jellyfish Aequorea Victoria with 238 amino acids residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Arigo ARG62344 GFP antibody recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP.

Target Name Tag Internal Control
Alternate Names Tag Internal Control antibody; GAPDH antibody; GFP antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Antibody Duo for Tag Internal Control (GFP, GAPDH)
Research Area Cancer antibody; Controls and Markers antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

Images (8) Click the Picture to Zoom In

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western blot: 1) U87-MG 2) HepG2 3) rat brain 4) rat heart 5) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:2000 dilution.

  • ARG10112 anti-GAPDH antibody [6C5] ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG10112 anti-GAPDH antibody [6C5] (green) at 1:200 dilution.

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western Blot: 1) HeLa, 2) NTERA-2, 3) A-431, 4) HepG2, 5) MCF-7, 6) NIH 3T3, 7) PC-12 and 8) COS-7 whole cell lysates stained with anti-GAPDH antibody [6C5] (ARG10112)

  • ARG62343 anti-GFP antibody [GF28R] WB image

    Western Blot: HEK293 cells transfected with GFP-tagged protein vector; (1) untransfected and (2) transfected stained with ARG62343 anti-GFP antibody [GF28R] at 1:1000 (1 μg/mL) dilution

  • ARG62344 anti-RFP antibody [RF5R] WB image

    Western Blot: HEK293 cells transfected with RFP-tagged protein vector; (1) untransfected and (2) transfected with Turbo-RFP (2), and (3) transfected with DsRed stained with ARG62344 anti-RFP antibody [RF5R] at 1:1000 (1 μg/mL) dilution

  • ARG10112 anti-GAPDH antibody [6C5] IP image

    Immunoprecipitation and western blot: 1) GAPDH (1 μg). 2) GAPDH IP from rat heart tissue extract. 3) Only GAPDH preincubated with Protein A Sepharose. 4) Only Protein A Sepharose stained with ARG10112 GAPDH antibody [6C5].


    Mixture of protein A-Sepharose with ARG10112 anti-GAPDH and tissue extract was incubated for 30 min at room temperature and precipitated by centrifugation. Pellet was washed with PBS, suspended in reducing electrophoresis sample buffer and heated for 5 minutes at 100ºC. After centrifugation supernatant was loaded on gel and proteins were separated by SDS electrophoresis.

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western blot: 1) U87-MG 2) MCF-7 3) A549 4) DU145 5) SW480 6) rat brain 7) rat stomach 8) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:5000 dilution.

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western blot: 1) MCF-7 2) DU-145 3) A549 4) H1299 5) HCT116 6) HepG2 7) HUVEC stained with ARG10112 anti-GAPDH antibody [6C5] at 1:1000 dilution.

Specific References

Downregulation of lncRNA NEAT1 alleviates sepsis-induced acute kidney injury

ARG10112: WB / Human

Yuhua Zhou et al.
Central European Journal of Immunology,  (2022)

publication_link

 

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Curcumin attenuates renal ischemia reperfusion injury via JNK pathway with the involvement of p300/CBP-mediated histone acetylation

ARG10112: WB / Rat

Lu Yang et al.
Korean J Physiol Pharmacol.,  (2021)

publication_link

 

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7,8-dihydroxyflavone ameliorates motor deficits via regulating autophagy in MPTP-induced mouse model of Parkinson's disease

ARG10112: WB / Mouse

Li Zuo et al.
Cell Death Discov.,  (2021)

publication_link

 

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Evaluation of Class IIa Histone Deacetylases Expression and In Vivo Epigenetic Imaging in a Transgenic Mouse Model of Alzheimer's Disease.

ARG10112: WB / Human

Yi-An Chen et al.
International Journal of Molecular Sciences.,  (2021)

publication_link

 

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Cardiac-Specific Gene TNNI3 as a Potential Oncogene for Kidney Cancer and Its Involvement in Wnt Signaling Pathway.

ARG10112: WB / Human

Weian Zhao et al.
Preprint.,  (2021)

publication_link

 

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Mesenchymal Stem Cell-Derived Exosomes Ameliorate Alzheimer's Disease Pathology and Improve Cognitive Deficits.

ARG10112: WB / Human

Yi-An Chen et al.
Biomedicines.,  (2021)

publication_link

 

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Circ_0081572 inhibits the progression of periodontitis through regulating the miR-378h/RORA axis.

ARG10112: WB / Human

Jing Wang et al.
Arch Oral Biol.,  (2021)

publication_link

 

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Structural Basis of VSIG3: The Ligand for VISTA.

ARG10112: WB / Human

Xiaoxue Xie et al.
Front Immunol.,  (2021)

publication_link

 

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The Phosphorylation Status of Drp1-Ser637 by PKA in Mitochondrial Fission Modulates Mitophagy via PINK1/Parkin to Exert Multipolar Spindles Assembly during Mitosis.

ARG10112: WB / Human

Huey-Jiun Ko et al.
Biomolecules.,  (2021)

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M351-0056 is a novel low MW compound modulating the actions of the immune-checkpoint protein VISTA.

ARG10112: WB / Human, Mouse

Xinlei Hu et al.
Br J Pharmacol.,  (2021)

publication_link

 

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SCM-198 protects endometrial stromal cells from oxidative damage through Bax/Bcl-2 and ERK signaling pathways.

ARG10112: WB / Human

Yunyun Li et al.
Acta Biochim Biophys Sin.,  (2019)

publication_link

 

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Excessive fibroblast growth factor 23 promotes renal fibrosis in mice with type 2 cardiorenal syndrome.

ARG10112: WB / Mouse, Rat

Huixin Hao et al.
Aging (Albany NY).,  (2021)

publication_link

 

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ERK activation precedes Purkinje cell loss in mice with Spinocerebellar ataxia type 17.

ARG10112: WB / Mouse

Chia-Wei Lin et al.
Neurosci Lett.,  (2020)

publication_link

 

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Synthesis and anti-inflammatory activity of saponin derivatives of δ-oleanolic acid.

ARG10112: WB / Human

Liu Liu et al.
European Journal of Medicinal Chemistry.,  (2020)

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SMARCB1 Promotes Ubiquitination and Degradation of NR4A3 via Direct Interaction Driven by ROS in Vascular Endothelial Cell Injury.

ARG10112: WB / Human, Rat

Bingzheng Lu et al.
Oxidative Medicine and Cellular Longevity.,  (2020)

publication_link

 

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Study of isolation and characterization of extracellular vesicles in senescent tumor cells induced by chemotherapeutics.

ARG10112: WB / Human

Qi Zhou et al.
Chinese Journal of Immunology.,  (2020)

publication_link

 

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Increased expression levels of inflammatory cytokines and adhesion molecules in lipopolysaccharide-induced acute inflammatory apoM-/- mice.

ARG10112: WB / Mouse

Yuanping Shi et al.
Molecular Medicine Reports.,  (2020)

publication_link

 

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Discovery of AdipoRon analogues as novel AMPK activators without inhibiting mitochondrial complex I.

ARG10112: WB / Human, Mouse

Geng Sun et al.
Eur J Med Chem.,  (2020)

publication_link

 

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Discovery of AdipoRon analogues as novel AMPK activators without inhibiting mitochondrial complex I.

ARG10112: WB / Human, Mouse

Sun Geng et al.
European Journal of Medicinal Chemistry.,  (2020)

publication_link

 

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Cardiac troponin I R193H mutant interacts with HDAC1 to repress phosphodiesterase 4D expression in cardiomyocytes.

ARG10112: WB / Mouse

Zhao Weian et al.
Genes & Diseases.,  (2020)

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Epigenetic regulation of phosphodiesterase 4d in restrictive cardiomyopathy mice with cTnI mutations.

ARG10112: WB / Mouse

Zhao Weian et al.
Sci China Life Sci.,  (2019)

publication_link

 

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Tumor-associated neutrophils induce EMT by IL-17a to promote migration and invasion in gastric cancer cells.

ARG10112: WB / Human

Li Sen et al.
J Exp Clin Cancer Res.,  (2019)

publication_link

 

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Genetic labeling reveals temporal and spatial expression pattern of D2 dopamine receptor in rat forebrain.

ARG10112: WB / Rat

Yu Qing et al.
Brain Struct Funct.,  (2019)

publication_link

 

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AdipoRon prevents l-thyroxine or isoproterenol-induced cardiac hypertrophy through regulating the AMPK-related pathway.

ARG10112: WB / Rat

Hu Xinlei et al.
Acta Biochim Biophys Sin (Shanghai).,  (2018)

publication_link

 

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The effect of melatonin on cardio fibrosis in juvenile rats with pressure overload and deregulation of HDACs.

ARG10112: WB / Rat

Wu Yao et al.
Korean J Physiol Pharmacol.,  (2018)

publication_link

 

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Epigallocatechin-3 gallate prevents pressure overload-induced heart failure by up-regulating SERCA2a via histone acetylation modification in mice.

ARG10112: WB / Mouse

Liu Lifei et al.
PLoS One.,  (2018)

publication_link

 

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Checking transfer efficiency and equal loading via qualitative optical way in western blotting.

ARG10112: WB / Human

Gong JH et al.
Electrophoresis,  (2017)

publication_link

 

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Cardiac Protection of Valsartan on Juvenile Rats with Heart Failure by Inhibiting Activity of CaMKII via Attenuating Phosphorylation

ARG10112: WB / Rat

Yao Wu et al.
BioMed Research International.,  (2017)

publication_link

 

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Hydrogen sulfide protects against endoplasmic reticulum stress and mitochondrial injury in nucleus pulposus cells and ameliorates intervertebral disc degeneration.

ARG10112: WB / Human

Xu D et al.
Pharmacol Res.,  (2017)

publication_link

 

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Acute hyperglycemia suppresses left ventricular diastolic function and inhibits autophagic flux in mice under prohypertrophic stimulation.

ARG10112: WB / Mouse

Xie J et al.
Cardiovasc Diabetol.,  (2016)

publication_link

 

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Reciprocal regulation between O-GlcNAcylation and tribbles pseudokinase 2 (TRIB2) maintains transformative phenotypes in liver cancer cells.

ARG10112: WB / Human

Yao B et al.
Cell Signal.,  (2016)

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