ARG10112

anti-GAPDH antibody [6C5]

anti-GAPDH antibody [6C5] for ELISA,ICC/IF,IHC-Frozen sections,Western blot and African green monkey,Baboon,Cat,Chicken,Dog,Fish,Hamster,Human,Monkey,Mouse,Pig,Rabbit,Rat,Xenopus laevis,Zebrafish

Cancer antibody; Controls and Markers antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Loading Control antibody; Loading Control antibody for Cytoplasmic Fractions; Organelle Marker antibody for Cytoplasm; Autophagy Study antibody
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Overview

Product Description

Mouse Monoclonal antibody [6C5] recognizes GAPDH

Tested Reactivity Hu, Ms, Rat, AGMK, Bb, Cat, Chk, Dog, Fsh, Hm, Mk, Pig, Rb, Xenopus laevis, Zfsh
Predict Reactivity Gpig, Hrs, Xenopus tropicalis
Species Does Not React With Bov, Goat, S. cerevisiae
Tested Application ELISA, ICC/IF, IHC-Fr, WB
Specificity This antibody is specific for GAPDH of Human, Porcine, Canine, Rabbit, Cat, Rat, Mouse, Fish.
Host Mouse
Clonality Monoclonal
Clone 6C5
Isotype IgG1
Target Name GAPDH
Antigen Species Rabbit
Immunogen Rabbit muscle GAPDH
Conjugation Un-conjugated
Alternate Names Glyceraldehyde-3-phosphate dehydrogenase; GAPD; HEL-S-162eP; G3PD; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH; EC 2.6.99.-; EC 1.2.1.12

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IF5 µg/ml
IHC-Fr1:500
WB1:1000 - 1:10000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa; NTERA-2; A-431; HepG2; MCF-; NIH 3T3; PC-12; COS-7

Properties

Form Liquid
Purification Protein A affinity purified.
Buffer PBS (pH 7.4) and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 100009074 Rabbit GAPDH

GeneID: 14433 Mouse GAPDH

GeneID: 24383 Rat GAPDH

Gene Symbol GAPDH
Gene Full Name glyceraldehyde-3-phosphate dehydrogenase
Background GAPDH protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
Function GAPDH has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. [UniProt]
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Research Area Cancer antibody; Controls and Markers antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Loading Control antibody; Loading Control antibody for Cytoplasmic Fractions; Organelle Marker antibody for Cytoplasm; Autophagy Study antibody
Calculated MW 36 kDa
PTM S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus (By similarity). S-nitrosylation of Cys-247 is induced by interferon-gamma and LDL(ox) implicating the iNOS-S100A8/9 transnitrosylase complex and seems to prevent interaction with phosphorylated RPL13A and to interfere with GAIT complex activity.
ISGylated.
Sulfhydration at Cys-152 increases catalytic activity.
Oxidative stress can promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process called nucleocytoplasmic coagulation. Such aggregates can be observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at Met-46 may play a pivotal role in the formation of these insoluble structures. This modification has been detected in vitro following treatment with free radical donor (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide. It has been proposed to destabilize nearby residues, increasing the likelihood of secondary oxidative damages, including oxidation of Tyr-45 and Met-105. This cascade of oxidations may augment GAPDH misfolding, leading to intermolecular disulfide cross-linking and aggregation.

Images (4) Click the Picture to Zoom In

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western blot: 1) U87-MG 2) HepG2 3) rat brain 4) rat heart 5) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:2000 dilution.

  • ARG10112 anti-GAPDH antibody [6C5] ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG10112 anti-GAPDH antibody [6C5] (green) at 1:200 dilution.

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western blot: 1) U87-MG 2) MCF-7 3) A549 4) DU145 5) SW480 6) rat brain 7) rat stomach 8) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:5000 dilution.

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western blot: 1) MCF-7 2) DU-145 3) A549 4) H1299 5) HCT116 6) HepG2 7) HUVEC stained with ARG10112 anti-GAPDH antibody [6C5] at 1:1000 dilution.

Customer's Feedback

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Review for anti-GAPDH antibody [6C5]

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:FITC

nuts_pic      Excellent

Review for anti-GAPDH antibody [6C5]

Application:WB

Sample:HaCaT cell

Sample Loading Amount:40μg

Primary Antibody Dilution Factor:1:10,000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Evaluation of Class IIa Histone Deacetylases Expression and In Vivo Epigenetic Imaging in a Transgenic Mouse Model of Alzheimer's Disease.

WB / Human

Yi-An Chen et al.
International Journal of Molecular Sciences.,  (2021)

publication_link

 

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Cardiac-Specific Gene TNNI3 as a Potential Oncogene for Kidney Cancer and Its Involvement in Wnt Signaling Pathway.

WB / Human

Weian Zhao et al.
Preprint.,  (2021)

publication_link

 

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Mesenchymal Stem Cell-Derived Exosomes Ameliorate Alzheimer's Disease Pathology and Improve Cognitive Deficits.

WB / Human

Yi-An Chen et al.
Biomedicines.,  (2021)

publication_link

 

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Circ_0081572 inhibits the progression of periodontitis through regulating the miR-378h/RORA axis.

WB / Human

Jing Wang et al.
Arch Oral Biol.,  (2021)

publication_link

 

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Structural Basis of VSIG3: The Ligand for VISTA.

WB / Human

Xiaoxue Xie et al.
Front Immunol.,  (2021)

publication_link

 

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The Phosphorylation Status of Drp1-Ser637 by PKA in Mitochondrial Fission Modulates Mitophagy via PINK1/Parkin to Exert Multipolar Spindles Assembly during Mitosis.

WB / Human

Huey-Jiun Ko et al.
Biomolecules.,  (2021)

publication_link

 

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M351-0056 is a novel low MW compound modulating the actions of the immune-checkpoint protein VISTA.

WB / Human, Mouse

Xinlei Hu et al.
Br J Pharmacol.,  (2021)

publication_link

 

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SCM-198 protects endometrial stromal cells from oxidative damage through Bax/Bcl-2 and ERK signaling pathways.

WB / Human

Yunyun Li et al.
Acta Biochim Biophys Sin.,  (2019)

publication_link

 

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Excessive fibroblast growth factor 23 promotes renal fibrosis in mice with type 2 cardiorenal syndrome.

WB / Mouse, Rat

Huixin Hao et al.
Aging (Albany NY).,  (2021)

publication_link

 

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ERK activation precedes Purkinje cell loss in mice with Spinocerebellar ataxia type 17.

WB / Mouse

Chia-Wei Lin et al.
Neurosci Lett.,  (2020)

publication_link

 

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Synthesis and anti-inflammatory activity of saponin derivatives of δ-oleanolic acid.

WB / Human

Liu Liu et al.
European Journal of Medicinal Chemistry.,  (2020)

publication_link

 

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SMARCB1 Promotes Ubiquitination and Degradation of NR4A3 via Direct Interaction Driven by ROS in Vascular Endothelial Cell Injury.

WB / Human, Rat

Bingzheng Lu et al.
Oxidative Medicine and Cellular Longevity.,  (2020)

publication_link

 

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Study of isolation and characterization of extracellular vesicles in senescent tumor cells induced by chemotherapeutics.

WB / Human

Qi Zhou et al.
Chinese Journal of Immunology.,  (2020)

publication_link

 

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Increased expression levels of inflammatory cytokines and adhesion molecules in lipopolysaccharide-induced acute inflammatory apoM-/- mice.

WB / Mouse

Yuanping Shi et al.
Molecular Medicine Reports.,  (2020)

publication_link

 

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Discovery of AdipoRon analogues as novel AMPK activators without inhibiting mitochondrial complex I.

WB / Human, Mouse

Geng Sun et al.
Eur J Med Chem.,  (2020)

publication_link

 

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Discovery of AdipoRon analogues as novel AMPK activators without inhibiting mitochondrial complex I.

WB / Human, Mouse

Sun Geng et al.
European Journal of Medicinal Chemistry.,  (2020)

publication_link

 

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Cardiac troponin I R193H mutant interacts with HDAC1 to repress phosphodiesterase 4D expression in cardiomyocytes.

WB / Mouse

Zhao Weian et al.
Genes & Diseases.,  (2020)

publication_link

 

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Epigenetic regulation of phosphodiesterase 4d in restrictive cardiomyopathy mice with cTnI mutations.

WB / Mouse

Zhao Weian et al.
Sci China Life Sci.,  (2019)

publication_link

 

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Tumor-associated neutrophils induce EMT by IL-17a to promote migration and invasion in gastric cancer cells.

WB / Human

Li Sen et al.
J Exp Clin Cancer Res.,  (2019)

publication_link

 

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Genetic labeling reveals temporal and spatial expression pattern of D2 dopamine receptor in rat forebrain.

WB / Rat

Yu Qing et al.
Brain Struct Funct.,  (2019)

publication_link

 

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AdipoRon prevents l-thyroxine or isoproterenol-induced cardiac hypertrophy through regulating the AMPK-related pathway.

WB / Rat

Hu Xinlei et al.
Acta Biochim Biophys Sin (Shanghai).,  (2018)

publication_link

 

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The effect of melatonin on cardio fibrosis in juvenile rats with pressure overload and deregulation of HDACs.

WB / Rat

Wu Yao et al.
Korean J Physiol Pharmacol.,  (2018)

publication_link

 

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Epigallocatechin-3 gallate prevents pressure overload-induced heart failure by up-regulating SERCA2a via histone acetylation modification in mice.

WB / Mouse

Liu Lifei et al.
PLoS One.,  (2018)

publication_link

 

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Checking transfer efficiency and equal loading via qualitative optical way in western blotting.

WB / Human

Gong JH et al.
Electrophoresis,  (2017)

publication_link

 

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Cardiac Protection of Valsartan on Juvenile Rats with Heart Failure by Inhibiting Activity of CaMKII via Attenuating Phosphorylation

WB / Rat

Yao Wu et al.
BioMed Research International.,  (2017)

publication_link

 

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Hydrogen sulfide protects against endoplasmic reticulum stress and mitochondrial injury in nucleus pulposus cells and ameliorates intervertebral disc degeneration.

WB / Human

Xu D et al.
Pharmacol Res.,  (2017)

publication_link

 

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Acute hyperglycemia suppresses left ventricular diastolic function and inhibits autophagic flux in mice under prohypertrophic stimulation.

WB / Mouse

Xie J et al.
Cardiovasc Diabetol.,  (2016)

publication_link

 

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Reciprocal regulation between O-GlcNAcylation and tribbles pseudokinase 2 (TRIB2) maintains transformative phenotypes in liver cancer cells.

WB / Human

Yao B et al.
Cell Signal.,  (2016)

publication_link

 

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