ARG30334

Tumor-infiltrating Lymphocyte Antibody Panel

Tumor infiltrating Lymphocyte markers; Immunology; anti-tumor immune responses; tumor immune infiltration

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG65859 anti-CD3 antibody [SQab1713] Rabbit mAb Hu FACS, ICC/IF, IHC-P, IP, WB 20 μl
ARG66628 anti-CD8 antibody [SQab19146] Rabbit mAb Hu IHC-P 20 μl
ARG65860 anti-CD4 antibody [SQab1714] Rabbit mAb Hu FACS, IHC-P, IP, WB 20 μl
ARG66197 anti-CD20 antibody [SQab1719] Rabbit mAb Hu FACS, ICC/IF, IHC-P, IP 20 μl

Overview

Product Description Tumor-infiltrating Lymphocyte (TIL) Antibody Panel is an all-in-one solution to make identification of TILs easy and economic. This antibody panel comprises antibodies against CD3, CD4, CD8 and CD20 for identifying general T cells, Treg cells, cytotoxic T cells and B cells, respectively. All the antibodies in this panel have excellent IHC staining performance.
Target Name Tumor-infiltrating Lymphocyte
Full Name Antibody Panel for Tumor-infiltrating Lymphocyte
Alternate Names Tumor-infiltrating Lymphocyte antibody; CD3 antibody; CD4 antibody; CD20 antibody; CD8 antibody

Properties

Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Antibody Panel for Tumor-infiltrating Lymphocyte
Highlight Related news:
New antibody panels and duos for Tumor immune microenvironment
Tumor-Infiltrating Lymphocytes (TILs)
Research Area Tumor infiltrating Lymphocyte markers; Immunology; anti-tumor immune responses; tumor immune infiltration

Images (16) Click the Picture to Zoom In

  • ARG65859 anti-CD3 antibody [SQab1713] WB image

    Western blot: 30 µg of Molt4 cell lysate stained with ARG65859 anti-CD3 antibody [SQab1713] at 1:500 dilution.

  • ARG65860 anti-CD4 antibody [SQab1714] WB image

    Western blot: 30 µg of Jurkat and Molt4 cell lysates stained with ARG65860 anti-CD4 antibody [SQab1714] at 1:500 dilution.

  • ARG65859 anti-CD3 antibody [SQab1713] ICC/IF image

    Immunofluorescence: Jurkat cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% Goat serum for half an hour at room temperature. Samples were stained with ARG65859 anti-CD3 antibody [SQab1713] (green) at 1:50 and 4°C. DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody.

  • ARG66197 anti-CD20 antibody [SQab1719] ICC/IF image

    Immunofluorescence: Raji cells fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% Goat serum for half an hour at room temperature. Samples were stained with ARG66197 anti-CD20 antibody [SQab1719] (green) at 1:1000 at 4°C. DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody.

  • ARG65859 anti-CD3 antibody [SQab1713] FACS image

    Flow Cytometry: Jurkat cells were fixed with 4% paraformaldehyde for 10 min. The cells were then stained with ARG65859 anti-CD3 antibody [SQab1713] (blue) at 1:1000 dilution in 1x PBS/1% BSA for 30 min at room temperture, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (red) was used as a control.

  • ARG65860 anti-CD4 antibody [SQab1714] FACS image

    Flow Cytometry: Jurkat cells were fixed with 4% paraformaldehyde for 10 min. The cells were then stained with ARG65860 anti-CD4 antibody [SQab1714] (blue) at 1:50 dilution in 1x PBS/1% BSA for 30 min at room temperture, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (red) was used as a control.

  • ARG66197 anti-CD20 antibody [SQab1719] FACS image

    Flow Cytometry: Raji cells were fixed with 4% paraformaldehyde for 10 min. The cells were then stained with ARG66197 anti-CD20 antibody [SQab1719] (red) at 1:500 dilution in 1x PBS/1% BSA for 30 min at room temperture, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.

  • ARG65859 anti-CD3 antibody [SQab1713] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human tonsil tissue stained with ARG65859 anti-CD3 antibody [SQab1713] at 1:200 dilution. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).

  • ARG65860 anti-CD4 antibody [SQab1714] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human tonsil tissue stained with ARG65860 anti-CD4 antibody [SQab1714] at 1:2000 dilution. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).

  • ARG66197 anti-CD20 antibody [SQab1719] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human appendix tissue stained with ARG66197 anti-CD20 antibody [SQab1719] at 1:20000 dilution.

    Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0

  • ARG66628 anti-CD8 antibody [SQab19146] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human tonsil tissue stained with ARG66628 anti-CD8 antibody [SQab19146]. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).

  • ARG65859 anti-CD3 antibody [SQab1713] IP image

    Immunoprecipitation: 0.4 mg of Molt-4 whole cell lysate was immunoprecipitated (1:15 dilution) and stained with ARG65859 anti-CD3 antibody [SQab1713].

    Lane 1: Immunoprecipitation in Molt-4 whole cell lysate
    Lane 2: Rabbit IgG instead of Primary Ab in Molt-4 whole cell lysate
    Lane 3: Molt-4 whole cell lysate, 10 µg (input)

  • ARG65860 anti-CD4 antibody [SQab1714] IP image

    Immunoprecipitation: 0.4 mg of Molt-4 whole cell lysate was immunoprecipitated (1:50 dilution) and stained with ARG65860 anti-CD4 antibody [SQab1714].

    Lane 1: Immunoprecipitation in Molt-4 whole cell lysate
    Lane 2: Rabbit IgG instead of Primary Ab in Molt-4 whole cell lysate
    Lane 3: Molt-4 whole cell lysate, 10 µg (input)

  • ARG66197 anti-CD20 antibody [SQab1719] IP image

    Immunoprecipitation: 0.4 mg of Raji whole cell lysate was immunoprecipitated (1:20 dilution) and stained with ARG66197 anti-CD20 antibody [SQab1719].

    Lane 1: Immunoprecipitation in Raji whole cell lysate
    Lane 2: PBS instead of Primary Ab in Raji whole cell lysate
    Lane 3: Raji whole cell lysate, 10 µg (input)

  • ARG65859 anti-CD3 antibody [SQab1713] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human colon tissue stained with ARG65859 anti-CD3 antibody [SQab1713] at 1:200 dilution. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).

  • ARG66197 anti-CD20 antibody [SQab1719] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human spleen tissue stained with ARG66197 anti-CD20 antibody [SQab1719] at 1:20000 dilution.

    Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0