ARG20531

anti-BiP / GRP78 antibody

anti-BiP / GRP78 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunohistochemistry (PFA perfusion fixed frozen sections),Immunoprecipitation,Western blot and Bovine,Dog,Fungi,Hamster,Human,Monkey,Mouse,Rabbit,Rat,Xenopus laevis

Controls and Markers antibody; ER Marker antibody; Endoplasmic reticulum antibody
nuts_pic
1
anti-BiP / GRP78 antibody

Rabbit Polyclonal antibody recognizes BiP / GRP78

ARG20531 Arigo biolaboratories
4.0 out of 5 based on 1
anti-BiP / GRP78 antibody is rated 4.0 out of 5 by 1.

anti-BiP / GRP78 antibody

publication_link Publication5

Overview

Product Description

Rabbit Polyclonal antibody recognizes BiP / GRP78

Tested Reactivity Hu, Ms, Rat, Bov, Dog, Fungi, Hm, Mk, Rb, Xenopus laevis
Predict Reactivity Bov, Hm, Mk, Rb, Xenopus
Tested Application ICC/IF, IHC-FoFr , IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name BiP / GRP78
Antigen Species Rat
Immunogen KLH-conjugated sythetic peptide of Rat BiP / GRP78.
Conjugation Un-conjugated
Full Name heat shock protein 5
Alternate Names Heat shock 70 kDa protein 5; BiP; GRP-78; Endoplasmic reticulum lumenal Ca; MIF2; 78 kDa glucose-regulated protein; GRP78; Immunoglobulin heavy chain-binding protein; HEL-S-89n; BIP; 2+

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
IHC-FoFr Assay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 72 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer Rabbit antiserum.
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 14828 Mouse HSPA5

GeneID: 25617 Rat HSPA5

GeneID: 3309 Human HSPA5

Gene Symbol Hspa5
Gene Full Name heat shock protein 5
Background The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]
Function Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate (By similarity). [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30316 ER Marker Antibody Duo
Related products:
GRP78 antibodies; GRP78 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Controls and Markers antibody; ER Marker antibody; Endoplasmic reticulum antibody

Images (5) Click the Picture to Zoom In

  • ARG20531 anti-BiP / GRP78 antibody ICC/IF image

    Immunofluorescence: Heat Shocked (42°C for 30 min) HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: ARG20531 anti-BiP / GRP78 antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain. Middle: ARG20531 anti-BiP / GRP78 antibody. Right: Composite.

  • ARG20531 anti-BiP / GRP78 antibody IHC image

    Immunohistochemistry: Formaldehdye-fixed and parrafin embedded sections of Mouse hippocampal tissue stained with ARG20531 anti-BiP / GRP78 antibody at 1:100 dilution. Tissue was counterstained using DAPI at a 1:1000 dilution in order to visualize the nuclei in the pyramidal cell layer. Images courtsey of Rachel Reith, NIH/NIMH.

  • ARG20531 anti-BiP / GRP78 antibody WB image

    Western blot: Human, Dog, Mouse cell line lysates stained with ARG20531 anti-BiP / GRP78 antibody at 1:1000 dilution.

  • ARG20531 anti-BiP / GRP78 antibody ICC/IF image

    Immunofluorescence: Heat Shocked (42°C for 30 min) HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: ARG20531 anti-BiP / GRP78 antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain. Middle: ARG20531 anti-BiP / GRP78 antibody. Right: Composite.

  • ARG20531 anti-BiP / GRP78 antibody WB image

    Western blot: Rat brain, heart, kidney, liver, lung, pancreas, skeletal muscle, spleen, testes, thymus, H9C2 cell, Mouse NIH 3T3 and Pam212 cell lysates stained with ARG20531 anti-BiP / GRP78 antibody.

Customer's Feedback

nuts_pic      Good

Review for anti-BiP / GRP78 antibody

Application:WB

Sample:3T3

Sample Loading Amount:20 µl

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Sp2 promotes invasion and metastasis of hepatocellular carcinoma by targeting TRIB3 protein.

WB, IHC-P / Human

Zhu Yue et al.
Cancer Med.,  (2020)

publication_link

 

hr_line

Resveratrol protects against CIH-induced myocardial injury by targeting Nrf2 and blocking NLRP3 inflammasome activation.

WB / Rat

Sun Zhi-Min et al.
Life Sci.,  (2020)

publication_link

 

hr_line

Interplay between Redox Signaling, Oxidative Stress, and Unfolded Protein Response (UPR) in Pathogenesis of Human Diseases.

WB / Rat

Poplawski Tomasz et al.
Oxid Med Cell Longev.,  (2019)

publication_link

 

hr_line

Hydrogen and Oxygen Mixture to Improve Cardiac Dysfunction and Myocardial Pathological Changes Induced by Intermittent Hypoxia in Rats.

WB / Rat

Zhao Ya-Shuo et al.
Oxidative Medicine and Cellular Longevity.,  (2019)

publication_link

 

hr_line

Regulation of P300 and HDAC1 on endoplasmic reticulum stress in isoniazid-induced HL-7702 hepatocyte injury.

WB / Human

Li Jin-Feng et al.
J Cell Physiol.,  (2019)

publication_link

 

hr_line