ARG20838

anti-CD11b antibody [3A33] (PE)

anti-CD11b antibody [3A33] (PE) for Blocking,Flow cytometry,ICC/IF and Mouse

MDSC Marker antibody; Myeloid-derived suppressor cell antibody

Overview

Product Description PE-conjugated Rat Monoclonal antibody [3A33] recognizes CD11b
Tested Reactivity Ms
Tested Application BL, FACS, ICC/IF
Specificity Mouse CD11b.
Host Rat
Clonality Monoclonal
Clone 3A33
Isotype IgG2a, kappa
Target Name CD11b
Species Mouse
Immunogen Peritoneal macrophages from B6D2 hybrid mice
Conjugation PE
Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Alternate Names MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
FACS< 0.3 µg/10^6 cells
ICC/IFAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 127 kDa

Properties

Form Liquid
Buffer PBS, Sodium azide and a stabilizing agent
Preservative Sodium azide
Concentration 0.1 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 16409 Mouse ITGAM

Gene Symbol ITGAM
Gene Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Background CD11b (integrin alpha M chain): Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Function CD11b: Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development. [UniProt]
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Research Area MDSC Marker antibody; Myeloid-derived suppressor cell antibody

Images (1) Click the Picture to Zoom In

  • ARG20838 anti-CD11b antibody [3A33] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG20838 anti-CD11b antibody [3A33] (PE) and ARG63064 anti-Ly6G + Ly6C antibody [RB6-8C5] (FITC).