ARG20851

anti-CD19 antibody [6D5] (Biotin)

anti-CD19 antibody [6D5] (Biotin) for Flow cytometry,IHC-Frozen sections and Mouse

Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; B cell Marker antibody; Pro-B Cell Marker antibody; Pre-B Cell Marker antibody; Immature B Cell Marker antibody; Follicular dendritic cells antibody

Overview

Product Description Biotin-conjugated Rat Monoclonal antibody [6D5] recognizes CD19
Tested Reactivity Ms
Tested Application FACS, IHC-Fr
Specificity Mouse CD19. The clone 6D5 recognizes the same epitope as the published clone, 1D3.
Host Rat
Clonality Monoclonal
Clone 6D5
Isotype IgG2a, kappa
Target Name CD19
Species Mouse
Immunogen Mouse CD19-expressing K562 human erythroleukemia cells
Conjugation Biotin
Full Name CD19 antigen
Alternate Names Differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; B4; CD antigen CD19; CVID3

Application Instructions

Application Suggestion
Tested Application Dilution
FACS< 1 µg/10^6 cells
IHC-FrAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 61 kDa

Properties

Form Liquid
Buffer PBS and Sodium azide
Preservative Sodium azide
Concentration 0.5 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 12478 Mouse CD19

Swiss-port # P25918 Mouse B-lymphocyte antigen CD19

Gene Symbol CD19
Gene Full Name CD19 antigen
Background CD19: Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]
Function CD19 functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed:2463100, PubMed:1373518, PubMed:16672701). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed:9382888, PubMed:9317126, PubMed:12387743, PubMed:16672701). Is not required for early steps during B cell differentiation in the blood marrow (PubMed:9317126). Required for normal differentiation of B-1 cells. Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:2463100, PubMed:1373518). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:9317126, PubMed:12387743, PubMed:16672701). [UniProt]
Highlight Related products:
CD19 antibodies; CD19 ELISA Kits; CD19 Duos / Panels; Anti-Rat IgG secondary antibodies;
Related news:
Tumor-Infiltrating Lymphocytes (TILs)
Research Area Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; B cell Marker antibody; Pro-B Cell Marker antibody; Pre-B Cell Marker antibody; Immature B Cell Marker antibody; Follicular dendritic cells antibody
PTM Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN.

Images (1) Click the Picture to Zoom In

  • ARG20851 anti-CD19 antibody [6D5] (Biotin) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG20851 anti-CD19 antibody [6D5] (Biotin) and ARG20819 anti-CD3e antibody [C363.29B] (PE) followed by Streptavidin (FITC).