ARG20819

anti-CD3e antibody [C363.29B] (PE)

anti-CD3e antibody [C363.29B] (PE) for Cellular activation ,Depletion,Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Mouse

Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody

Overview

Product Description PE-conjugated Rat Monoclonal antibody [C363.29B] recognizes CD3e
Tested Reactivity Ms
Tested Application Cell-Act , Depletion, FACS, IHC-Fr, IHC-P
Specificity Mouse CD3ε. The clone C363.29B recognizes an epitope on the 25 kDa ε chain of the CD3/TCR complex. In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naïve T cell in vitro. Immobilized C363.2B9 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present.
Host Rat
Clonality Monoclonal
Clone C363.29B
Isotype IgG2b, kappa
Target Name CD3e
Antigen Species Mouse
Immunogen IL-4 producing Th2 cell lines including D10
Conjugation PE
Full Name CD3 antigen, epsilon polypeptide
Alternate Names T-cell surface antigen T3/Leu-4 epsilon chain; T3E; TCRE; T-cell surface glycoprotein CD3 epsilon chain; IMD18; CD antigen CD3e

Application Instructions

Application Suggestion
Tested Application Dilution
Cell-Act Assay-dependent
DepletionAssay-dependent
FACS< 1 µg/10^6 cells
IHC-FrAssay-dependent
IHC-PAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 23 kDa

Properties

Form Liquid
Buffer PBS, Sodium azide and a stabilizing agent
Preservative Sodium azide
Concentration 0.1 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 12501 Mouse CD3E

Swiss-port # P22646 Mouse T-cell surface glycoprotein CD3 epsilon chain

Gene Symbol CD3E
Gene Full Name CD3 antigen, epsilon polypeptide
Background CD3 subunit complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Function CD3: Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). [UniProt]
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CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; Anti-Rat IgG secondary antibodies;
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Tumor-Infiltrating Lymphocytes (TILs)
Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody

Images (15) Click the Picture to Zoom In

  • ARG20819 anti-CD3e antibody [C363.29B] (PE) FACS image

    Flow Cytometry: BALB/c Mouse thymocytes stained with ARG22008 anti-CD28 antibody [37.51] (FITC) and ARG20819 anti-CD3e antibody [C363.29B] (PE).

  • ARG20819 anti-CD3e antibody [C363.29B] (PE) FACS image

    Flow Cytometry: BALB/c Mouse thymocytes stained with ARG22009 anti-CD28 antibody [37.51] (Biotin) and ARG20819 anti-CD3e antibody [C363.29B] (PE).

  • ARG20819 anti-CD3e antibody [C363.29B] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG22026 anti-CD45R / B220 antibody [RA3-6B2] (Biotin) and ARG20819 anti-CD3e antibody [C363.29B] (PE).

  • ARG20819 anti-CD3e antibody [C363.29B] (PE) FACS image

    Flow Cytometry: C57BL/6 Mouse splenocytes stained with ARG22027 anti-CD45R / B220 antibody [RA3-6B2] (Spectral Red) and ARG20819 anti-CD3e antibody [C363.29B] (PE).

  • ARG20819 anti-CD3e antibody [C363.29B] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG22029 anti-CD45R / B220 antibody [RA3-6B2] (PE/Cy5.5) and ARG20819 anti-CD3e antibody [C363.29B] (PE).

  • ARG20819 anti-CD3e antibody [C363.29B] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG22030 anti-CD45R / B220 antibody [RA3-6B2] (PE-Cy7) and ARG20819 anti-CD3e antibody [C363.29B] (PE).

  • ARG20819 anti-CD3e antibody [C363.29B] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG22039 anti-CD45RC antibody [GL24] (Biotin) and ARG20819 anti-CD3e antibody [C363.29B] (PE).

  • ARG20819 anti-CD3e antibody [C363.29B] (PE) FACS image

    Flow Cytometry: AKR Mouse splenocytes stained with ARG22101 anti-MHC Class II I-Ek antibody [17-3-3S] (FITC) and ARG20819 anti-CD3e antibody [C363.29B] (PE).

  • ARG20819 anti-CD3e antibody [C363.29B] (PE) FACS image

    Flow Cytometry: AKR Mouse splenocytes stained with ARG22126 anti-MHC Class I H-2Kk antibody [36-7-5] (FITC) and ARG20819 anti-CD3e antibody [C363.29B] (PE).

  • ARG20819 anti-CD3e antibody [C363.29B] (PE) FACS image

    Flow Cytometry: SJL Mouse splenocytes stained with ARG21013 anti-CD45 antibody [A20] (PE-Cy7) and ARG20819 anti-CD3e antibody [C363.29B] (PE).

  • ARG20819 anti-CD3e antibody [C363.29B] (PE) FACS image

    Flow Cytometry: C57BL/6 Mouse splenocytes stained with ARG21064 anti-MHC Class II I Ab antibody [25-9-3] (Fluorescein-5-maleimide) and ARG20819 anti-CD3e antibody [C363.29B] (PE).

  • ARG20819 anti-CD3e antibody [C363.29B] (PE) FACS image

    Flow Cytometry: AKR Mouse splenocytes stained with ARG21066 anti-MHC class II I E kappa antibody [14-4-4S] (FITC) and ARG20819 anti-CD3e antibody [C363.29B] (PE).

  • ARG20819 anti-CD3e antibody [C363.29B] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21070 anti-Anti-MHC Class II I Ad antibody [34-5-3] and ARG20819 anti-CD3e antibody [C363.29B] (PE).

  • ARG20819 anti-CD3e antibody [C363.29B] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG22333 anti-IL2 Receptor beta antibody [5H4] (FITC) and ARG20819 anti-CD3e antibody [C363.29B] (PE).

  • ARG20819 anti-CD3e antibody [C363.29B] (PE) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG20819 anti-CD3e antibody [C363.29B] (PE) and ARG20850 anti-CD19 antibody [6D5] (FITC).