ARG21114

anti-CD3e antibody [PPT3] (PE)

anti-CD3e antibody [PPT3] (PE) for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Pig

Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [PPT3] recognizes CD3e
Tested Reactivity Pig
Tested Application FACS, IHC-Fr, IHC-P, WB
Specificity Porcine CD3ε. The clone PPT3 is mitogenic when presented to peripheral blood mononuclear cells in immobilized form.
Host Mouse
Clonality Monoclonal
Clone PPT3
Isotype IgG1, kappa
Target Name CD3e
Species Pig
Immunogen Purified CD3 molecules from porcine thymus
Conjugation PE
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Alternate Names T-cell surface antigen T3/Leu-4 epsilon chain; T3E; TCRE; T-cell surface glycoprotein CD3 epsilon chain; IMD18; CD antigen CD3e

Application Instructions

Application Suggestion
Tested Application Dilution
FACS< 0.2 µg/10^6 cells
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 23 kDa

Properties

Form Liquid
Buffer PBS, Sodium azide and a stabilizing agent
Preservative Sodium azide
Concentration 0.1 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 397455 Pig CD3E

Swiss-port # Q7YRN2 Pig T-cell surface glycoprotein CD3 epsilon chain

Gene Symbol CD3E
Gene Full Name CD3e molecule, epsilon (CD3-TCR complex)
Background CD3 subunit complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Function CD3: Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). [UniProt]
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Tumor-Infiltrating Lymphocytes (TILs)
Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody

Images (3) Click the Picture to Zoom In

  • ARG21114 anti-CD3e antibody [PPT3] (PE) FACS image

    Flow Cytometry: Porcine peripheral blood lymphocytes stained with ARG21114 anti-CD3e antibody [PPT3] (PE) and ARG21121 anti-CD4 antibody [74-12-4] (FITC).

  • ARG21114 anti-CD3e antibody [PPT3] (PE) FACS image

    Flow Cytometry: Porcine small lymphocytes, monocytes, and granulocytes stained with ARG21132 anti-Monocyte + Granulocyte antibody [74-22-15] (Biotin) and ARG21114 anti-CD3e antibody [PPT3] (PE).

  • ARG21114 anti-CD3e antibody [PPT3] (PE) FACS image

    Flow Cytometry: Porcine peripheral blood lymphocytes stained with ARG21136 anti-CD21 antibody [BB6-11C9.6] (Biotin) and ARG21114 anti-CD3e antibody [PPT3] (PE).