ARG21147

anti-CD4 antibody [3-4F4] (FITC)

anti-CD4 antibody [3-4F4] (FITC) for Flow cytometry,IHC-Frozen sections,ICC/IF,Blocking and Cat

Developmental Biology antibody; Immune System antibody; Regulatory T cells Study antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [3-4F4] recognizes CD4
Tested Reactivity Cat
Tested Application BL, FACS, ICC/IF, IHC-Fr
Specificity Feline/Lion CD4.
Host Mouse
Clonality Monoclonal
Clone 3-4F4
Isotype IgG1, kappa
Target Name CD4
Species Cat
Immunogen Feline T cells
Conjugation FITC
Full Name CD4 molecule
Alternate Names CD4mut; CD antigen CD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
FACS< 1 µg/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 51 kDa

Properties

Form Liquid
Buffer PBS and Sodium azide
Preservative Sodium azide
Concentration 0.5 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CD4
Gene Full Name CD4 molecule
Background CD4 is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]
Function CD4 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. [UniProt]
Highlight Related products:
CD4 antibodies; CD4 ELISA Kits; CD4 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
New antibody panels and duos for Tumor immune microenvironment
Tumor-Infiltrating Lymphocytes (TILs)
Research Area Developmental Biology antibody; Immune System antibody; Regulatory T cells Study antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody
PTM Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.

Images (1) Click the Picture to Zoom In

  • ARG21147 anti-CD4 antibody [3-4F4] (FITC) FACS image

    Flow Cytometry: Feline peripheral blood lymphocytes stained with ARG21145 anti-CD8 antibody [fCD8] (PE) and ARG21147 anti-CD4 antibody [3-4F4] (FITC).