ARG62912

anti-CD63 antibody [MEM-259]

anti-CD63 antibody [MEM-259] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [MEM-259] recognizes CD63
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Specificity The clone MEM-259 reacts with CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.
Host Mouse
Clonality Monoclonal
Clone MEM-259
Isotype IgG1
Target Name CD63
Immunogen HPB-ALL T cell line
Conjugation Un-conjugated
Alternate Names Tspan-30; CD63 antigen; Tetraspanin-30; CD antigen CD63; Lysosomal-associated membrane protein 3; OMA81H; Ocular melanoma-associated antigen; Granulophysin; TSPAN30; Melanoma-associated antigen ME491; MLA1; LAMP-3; ME491

Application Instructions

Application Suggestion
Tested Application Dilution
FACS2 µg/ml
ICC/IFAssay-dependent
IHC-P10 µg/ml
IPAssay-dependent
WB0.5 - 10 µg/ml
Application Note WB: For non-reducing conditions only.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control FACS: Detection of activated platelets, neutrophils and basophils.
IHC-P: Spleen tissue.

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 967 Human CD63

Swiss-port # P08962 Human CD63 antigen

Gene Symbol CD63
Gene Full Name CD63 molecule
Background CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.
Function Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli. [UniProt]
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Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Immune System antibody
Calculated MW 26 kDa
PTM Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro).

Images (5) Click the Picture to Zoom In

  • ARG62912 anti-CD63 antibody [MEM-259] IHC-P image

    Immunohistochemistry: Paraffin-embedded Human spleen tissue stained with ARG62912 anti-CD63 antibody [MEM-259].

  • ARG62912 anti-CD63 antibody [MEM-259] ICC/IF image

    Immunofluorescence: Human skin fibroblasts stained with ARG62912 anti-CD63 antibody [MEM-259] (green).
    Co-stained with Transferrin (red) and DAPI (blue).

  • ARG62912 anti-CD63 antibody [MEM-259] ICC/IF image

    Immunofluorescence: Human primary fibroblasts stained with ARG62912 anti-CD63 antibody [MEM-259] (green).
    Actin cytoskeleton was stained with phalloidin (red) and cell nuclei stained with DAPI (blue).

  • ARG62912 anti-CD63 antibody [MEM-259] ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG62912 anti-CD63 antibody [MEM-259] (green).
    Actin cytoskeleton was stained with phalloidin (red) and cell nuclei stained with DAPI (blue).

  • ARG62912 anti-CD63 antibody [MEM-259] FACS image

    Flow Cytometry: IgE-activated Human peripheral blood stained with ARG62912 anti-CD63 antibody [MEM-259], followed by incubation with APC labelled Goat anti-Mouse secondary antibody.

Clone References

The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling.

IHC / Human

Van Deun J et al.
J Extracell Vesicles.,  (2014)

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Investigation of soluble and transmembrane CTLA-4 isoforms in serum and microvesicles.

EM / Human

Esposito L et al.
J Immunol.,  (2014)

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Epstein-Barr virus-encoded small RNAs (EBERs) are present in fractions related to exosomes released by EBV-transformed cells.

WB / Human

Ahmed W et al.
PLoS One.,  (2014)

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A human blood-brain barrier transcytosis assay reveals antibody transcytosis influenced by pH-dependent receptor binding.

ICC/IF / Human

Sade H et al.
PLoS One.,  (2014)

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Syntenin-ALIX exosome biogenesis and budding into multivesicular bodies are controlled by ARF6 and PLD2.

Ghossoub R et al.
Nat Commun.,  (2014)

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