ARG62934

anti-CD81 antibody [M38]

anti-CD81 antibody [M38] for Flow cytometry,Functional study,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Cat,Human,Rabbit

Immune System antibody; Microbiology and Infectious Disease antibody

Overview

Product Description

Mouse Monoclonal antibody [M38] recognizes CD81

Tested Reactivity Hu, Cat, Rb
Tested Application FACS, FuncSt, ICC/IF, IHC-P, IP, WB
Specificity The clone M38 reacts with CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.
Host Mouse
Clonality Monoclonal
Clone M38
Isotype IgG1
Target Name CD81
Antigen Species Human
Immunogen MOLT-4 (human T-ALL cell line)
Conjugation Un-conjugated
Alternate Names CD antigen CD81; TAPA1; Tspan-28; S5.7; CD81 antigen; Target of the antiproliferative antibody 1; Tetraspanin-28; 26 kDa cell surface protein TAPA-1; CVID6; TSPAN28

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 µg/ml
FuncStAssay-dependent
ICC/IF1:25 - 1:200
IHC-PAssay-dependent
IPAssay-dependent
WB1 - 2 µg/ml
Application Note Functional Application: In human MOLT-4 T-cell line the antibody M38 inhibits syncytium formation induced by co-culturing with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 975 Human CD81

Swiss-port # P60033 Human CD81 antigen

Gene Symbol CD81
Gene Full Name CD81 molecule
Background CD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T and other cells – e.g. in muscles CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus.
Function May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV. [UniProt]
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Research Area Immune System antibody; Microbiology and Infectious Disease antibody
Calculated MW 26 kDa
PTM Not glycosylated.

Images (6) Click the Picture to Zoom In

  • ARG62934 anti-CD81 antibody [M38] ICC/IF image

    Immunofluorescence: Huh7.5 cells were fixed with 3% PFA, permeabilized by 0.05% saponin and blocked with 0.5% BSA/PBS. Cells were stained with ARG62934 anti-CD81 antibody [M38] for 1 hour at RT.

  • ARG62934 anti-CD81 antibody [M38] WB image

    Western blot: Jurkat cell lysate stained with ARG62934 anti-CD81 antibody [M38] at 2 µg/ml dilution, under non-reducing condition.

  • ARG62934 anti-CD81 antibody [M38] WB image

    Western blot: K562 cell lysate stained with ARG62934 anti-CD81 antibody [M38] at 1:1000 dilution, under non-reducing condition.

  • ARG62934 anti-CD81 antibody [M38] FACS image

    Flow Cytometry: Human peripheral blood stained with ARG62934 anti-CD81 antibody [M38] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

  • ARG62934 anti-CD81 antibody [M38] WB image

    Western blot: Extracellular vesicles isolated from K562 cells. Sample was stained with ARG62934 anti-CD81 antibody [M38] at 1:1000 dilution, under non-reducing condition.

  • ARG62934 anti-CD81 antibody [M38] FACS image

    Flow Cytometry: Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG62934 anti-CD81 antibody [M38] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

Clone References

Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes.

Escola JM et al.
J Biol Chem.,  (1998)

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C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells.

Imai T et al.
J Immunol.,  (1993)

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Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells.

Fukudome K et al.
J Virol.,  (1992)

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