anti-CD9 antibody [MEM-61]

anti-CD9 antibody [MEM-61] for CyTOF®-candidate,Flow cytometry,Functional study,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody


Product Description Mouse Monoclonal antibody [MEM-61] recognizes CD9
Tested Reactivity Hu
Tested Application CyTOF®-candidate, FACS, FuncSt, IHC-P, WB
Specificity The clone MEM-61 recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
HLDA VI; WS Code P P-15
Host Mouse
Clonality Monoclonal
Clone MEM-61
Isotype IgG1
Target Name CD9
Immunogen Pre-B cell line NALM-6.
Conjugation Un-conjugated
Alternate Names Leukocyte antigen MIC3; BTCC-1; TSPAN-29; MIC3; Tetraspanin-29; p24; Cell growth-inhibiting gene 2 protein; CD9 antigen; MRP-1; DRAP-27; 5H9 antigen; CD antigen CD9; TSPAN29; Motility-related protein; Tspan-29

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
IHC-P20 µg/ml
WB2 - 4 µg/ml
Application Note WB: Under non-reducing condition.
Functional studies: The clone MEM-61 induces FcγR-dependent platelet aggregation.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control IHC-P: Prostate


Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 928 Human CD9

Swiss-port # P21926 Human CD9 antigen

Gene Symbol CD9
Gene Full Name CD9 molecule
Background CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.
Function Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. [UniProt]
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Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody
Calculated MW 25 kDa
PTM Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). The protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.

Images (1) Click the Picture to Zoom In

  • ARG62943 anti-CD9 antibody [MEM-61] CyTOF image

    CyTOF: PBMC (after Ficoll-Paque separation) stained with ARG62943 anti-CD9 antibody [MEM-61] (Dy161).

Clone References

A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration.

WB / Human

Salomon C et al.
PLoS One.,  (2014)




Hypoxia-induced changes in the bioactivity of cytotrophoblast-derived exosomes.

WB / Human

Salomon C et al.
PLoS One.,  (2013)




Sperm attachment and penetration competence in the human oocyte: a possible aetiology of fertilization failure involving the organization of oolemmal lipid raft microdomains influenced by the ΔΨm of subplasmalemmal mitochondria.

ICC/IF / Human

Van Blerkom J et al.
Reprod Biomed Online.,  (2013)




Role of interleukin 16 in multiple myeloma.

BL / Human

Atanackovic D et al.
J Natl Cancer Inst.,  (2012)