ARG20001

anti-Caspase 3 antibody [C33]

anti-Caspase 3 antibody [C33] for Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
nuts_pic
3
anti-Caspase 3 antibody [C33] Mouse Monoclonal antibody [C33] recognizes Caspase 3 ARG20001 Arigo biolaboratories
4.0 out of 5 based on 3
anti-Caspase 3 antibody [C33] is rated 4.0 out of 5 by 3.

anti-Caspase 3 antibody [C33]

publication_link Publication1

Overview

Product Description Mouse Monoclonal antibody [C33] recognizes Caspase 3
Tested Reactivity Hu
Tested Application WB
Specificity The antibody recognizes both proform and the cleaved large fragment of caspase-3 in samples of human, mouse and rat origins.
Host Mouse
Clonality Monoclonal
Clone C33
Isotype IgG1
Target Name Caspase 3
Antigen Species Human
Immunogen Recombinant human Caspase-3
Conjugation Un-conjugated
Alternate Names CPP-32; Caspase-3; EC 3.4.22.56; Apopain; CASP-3; CPP32; Cysteine protease CPP32; SCA-1; SREBP cleavage activity 1; CPP32B; Protein Yama

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.5-4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 32 kDa

Properties

Form Liquid
Purification Affinity Purified Antibody
Buffer PBS, 50% Glycerol, 0.5 mg/ml BSA and 0.01% Thimerosal
Preservative 0.01% Thimerosal
Stabilizer 50% Glycerol, 0.5 mg/ml BSA
Concentration 0.2 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 836 Human CASP3

Swiss-port # P42574 Human Caspase-3

Background The caspase family of cysteine proteases play a key role in apoptosis. Caspase-3 is the most extensively studied apoptotic protein among caspase family members. Caspase-3 is synthesized as inactive pro-enzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kD) and small (12 kD) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP and DFF).
Highlight Related Antibody Duos and Panels:
ARG30105 Apoptosis Marker Antibody Duo (Caspase3, PARP)
ARG30110 Mitochondria/Caspase dependant Apoptosis Antibody Panel (Caspase3, Caspase9, Cytochrome c, PARP) (WB)
Related products:
Caspase 3 antibodies; Caspase 3 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
PTM Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.

Images (2) Click the Picture to Zoom In

  • ARG20001 anti-Caspase 3 antibody [C33] WB image

    Western blot: 30 µg of HeLa cells untreated or treated with Staurosporine (1μM, overnight). The blots were stained with ARG20001 anti-Caspase 3 antibody [C33] at 1:500 dilution.

  • ARG20001 anti-Caspase 3 antibody [C33] WB image

    Western blot: 20 µg of HeLa cell lysates stained with ARG20001 anti-Caspase 3 antibody [C33] at 2 µg/ml dilution.

Customer's Feedback

nuts_pic      Good

Review for anti-Caspase 3 antibody [C33]

Application:WB

Sample:HeLa untreated / treated with Staurosporine (1μM, overnight)

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

Review for anti-Caspase 3 antibody [C33]

Application:WB

Sample:HeLa without/with Staurosporine

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

Review for anti-Caspase 3 antibody [C33]

Application:WB

Sample:PC-3

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:4 µg/ml

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Inhibition of histone acetylation by curcumin reduces alcohol-induced fetal cardiac apoptosis.

WB / Mouse (Since arigo failed mouse sample testing, therefore, the specie used in this paper is not covered the arigo's guarantee.)

Yan X et al.
J Biomed Sci.,  (2017)

publication_link

 

hr_line