ARG54155

anti-Caspase 9 antibody

anti-Caspase 9 antibody for Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
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Overview

Product Description Mouse Monoclonal antibody recognizes Caspase 9
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Mouse
Clonality Monoclonal
Target Name Caspase 9
Antigen Species Human
Immunogen Purified recombinant human Caspase-9 protein fragments expressed in E.coli.
Conjugation Un-conjugated
Alternate Names APAF-3; ICE-LAP6; PPP1R56; CASP-9; Apoptotic protease-activating factor 3; Caspase-9; ICE-like apoptotic protease 6; Apoptotic protease Mch-6; APAF3; MCH6; EC 3.4.22.62

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified
Buffer PBS (pH 7.4), 0.02% Sodium azide, and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 8 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12371 Mouse CASP9

GeneID: 58918 Rat CASP9

GeneID: 842 Human CASP9

Gene Symbol CASP9
Gene Full Name caspase 9, apoptosis-related cysteine peptidase
Background Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Function Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. [UniProt]
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Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
Calculated MW 46 kDa
PTM Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage.

Images (2) Click the Picture to Zoom In

  • ARG54155 anti-Caspase 9 antibody WB image

    Western blot: 20 µg of HeLa untreated or treated with ARG54155 anti-Caspase 9 antibody at 1:1000 dilution.

  • ARG54155 anti-Caspase 9 antibody WB image

    Western blot: 10 µg of HeLa, 20 µg of Mouse brain and 20 µg of Rat brain lysates stained with ARG54155 anti-Caspase 9 antibody at 1:1000 dilution.

Customer's Feedback

nuts_pic      Excellent

Review for anti-Caspase 9 antibody

Application:WB

Sample:HeLa, Mouse brain and Rat brain

Sample Loading Amount:10 µg of HeLa and 20 µg of brain lysates

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-Caspase 9 antibody

Application:WB

Sample:HeLa untreated/treated with Staurosporine

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-Caspase 9 antibody

Application:WB

Sample:1) Mouse brain, 2) Rat brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

Review for anti-Caspase 9 antibody

Application:WB

Sample:Jurkat

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC