ARG10002

anti-TGF beta antibody [TB21]

anti-TGF beta antibody [TB21] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Neutralizing,Western blot and Human,Mouse,Rat,Sheep

Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Metabolism antibody; Signaling Transduction antibody
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anti-TGF beta antibody [TB21]

Mouse Monoclonal antibody [TB21] recognizes TGF beta

ARG10002 Arigo biolaboratories
4.0 out of 5 based on 2
anti-TGF beta antibody [TB21] is rated 4.0 out of 5 by 2.

anti-TGF beta antibody [TB21]

Overview

Product Description

Mouse Monoclonal antibody [TB21] recognizes TGF beta

Tested Reactivity Hu, Ms, Rat, Sheep
Tested Application ELISA, ICC/IF, IHC-P, IHC-Fr, Neut, WB
Host Mouse
Clonality Monoclonal
Clone TB21
Isotype IgG1, k
Target Name TGF beta
Antigen Species Human
Immunogen TGF-β from human platelets
Conjugation Un-conjugated
Full Name transforming growth factor, beta 1
Alternate Names TGFB; DPD1; TGFbeta; CED; Transforming growth factor beta-1; LAP; TGF-beta-1

Application Instructions

Predict Reactivity Cow, Mink, Pig, Rb
Application Suggestion
Application Dilution
ELISAAssay-dependent
ICC/IFAssay-dependent
IHC-P1:1000
IHC-FrAssay-dependent
NeutAssay-dependent
WB1 - 2 µg/ml
Application Note Neutralizing: This antibody neutralizes TGF-β activity in vitro and in vivo. In an inhibition assay of CCL/64 cell growth and in a NRK-49F colony forming assay, the antibody neutralized TGF-β bioactivities. The effect of micro-injection of this antibody into one blastomere of two cell stage Xenopus embryos indicated that it was also able to neutralize the bioactivity of TGF-β in vivo.

IHC: This antibody has been used to demonstrate TGF-β in ovine ovarian tissue and human breast carcinoma at a dilution of 1:1000. As a consequence of the intense staining of the erythrocytes, it is possible to locate a single cell within the ovarian stroma, making it useful in locating very fine capillary networks within tissue.

Western Blot: This antibody when used at concentration of 5-20 ng/mL will allow visualization of 100 ng/lane of TGF-beta.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.0)
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 21803 Mouse TGFB1

GeneID: 59086 Rat TGFB1

GeneID: 7040 Human TGFB1

Gene Symbol TGFB1
Background This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.[provided by RefSeq, Oct 2009]
Function Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. [UniProt]
Resrarch Area Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Metabolism antibody; Signaling Transduction antibody
PTM Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.

Images (2) Click the Picture to Zoom In

  • ARG10002 anti-TGF beta antibody [TB21] WB image

    Western blot: 30 µg of Rat spleen and Rat heart lysates stained with ARG10002 anti-TGF beta antibody [TB21] at 1:1000 dilution.

  • ARG10002 anti-TGF beta antibody [TB21] WB image

    Western Blot: 1. Platelet-derived TGF-b (12 μg/lane), 2. Platelet-derived TGF-b (6 μg/lane), 3. Platelet-derived TGF-b (3 μg/lane), 4. Platelet-derived TGF-b (1 μg/lane), stained with anti-TGF beta antibody [TB21] (ARG10002) at 2µg/mL

    The bottom band is the 12.5KD TGF-b monomer, the mid-band is the 25KD TGF-b dimer and the up band is TGF-b with signal peptide. Lane 1 is molecular weight markers.

Customer's Feedback

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Review for anti-TGF beta antibody [TB21]

Application:WB

Sample:Rat spleen and Rat heart

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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nuts_pic      Good

Review for anti-TGF beta antibody [TB21]

Application:WB

Sample:Mouse spleen

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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Clone References

A functional polymorphism C-509T in TGFβ-1 promoter contributes to susceptibility and prognosis of lone atrial fibrillation in Chinese population.

WB / Human

Cao H et al.
PLoS One.,  (2014)

publication_link

 

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Fetal reprogramming and senescence in hypoplastic left heart syndrome and in human pluripotent stem cells during cardiac differentiation.

IHC-P / 

Gaber N et al.
Am J Pathol.,  (2013)

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Platelet-rich plasma promotes the proliferation of human muscle derived progenitor cells and maintains their stemness.

Neut / 

Li H et al.
PLoS One.,  (2013)

publication_link

 

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Losartan prevents heart fibrosis induced by long-term intensive exercise in an animal model.

WB / Rat

Gay-Jordi G et al.
PLoS One.,  (2013)

publication_link

 

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Ingredients of Huangqi decoction slow biliary fibrosis progression by inhibiting the activation of the transforming growth factor-beta signaling pathway.

IHC-Fr / Rat

Du JX et al.
BMC Complement Altern Med.,  (2012)

publication_link

 

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Development of a preclinical model of ischemic cardiomyopathy in swine.

WB / Pig

Ishikawa K et al.
Am J Physiol Heart Circ Physiol.,  (2011)

publication_link

 

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Immunotherapy for human renal cell carcinoma by adoptive transfer of autologous transforming growth factor beta-insensitive CD8+ T cells.

ICC/IF, IHC-P / Human

Wang L et al.
Clin Cancer Res.,  (2010)

publication_link

 

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