ARG20006

anti-Cytochrome C antibody

anti-Cytochrome C antibody for Immunoprecipitation,Western blot and Bovine,Chicken,Dog,Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody; Mitochondrial Marker antibody; Cytochrome-C fractionation Study antibody
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anti-Cytochrome C antibody

Rabbit Polyclonal antibody recognizes Cytochrome C

ARG20006 Arigo biolaboratories
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anti-Cytochrome C antibody is rated 4.0 out of 5 by 1.

anti-Cytochrome C antibody

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Overview

Product Description

Rabbit Polyclonal antibody recognizes Cytochrome C

Tested Reactivity Hu, Ms, Rat, Bov, Chk, Dog
Tested Application IP, WB
Specificity The cytochrome c antibody detects the 12.6 kDa cytochrome c from human, mouse, and rat samples.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Cytochrome C
Antigen Species Rat
Immunogen Synthetic peptide corresponding to residues surrounding amino acid 70 of rat cytochrome C
Conjugation Un-conjugated
Full Name cytochrome c, testis
Alternate Names CYCTA

Application Instructions

Application Suggestion
Tested Application Dilution
IP10-20 µg/ml
WB0.5-4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Jurkat cell lysate, NIH3T3 cell lysate, and rat kidney tissue lysate
Calculated MW 12 kDa

Properties

Form Liquid
Purification Affinity Purified Antibody
Buffer PBS (pH 7.2), 30% Glycerol, 0.5% BSA and 0.01% Thimerosal
Preservative 0.01% Thimerosal
Stabilizer 30% Glycerol, 0.5% BSA
Concentration 0.2 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 25310 Rat Cyct

Swiss-port # P10715 Rat Cytochrome c, testis-specific

Gene Symbol Cyct
Gene Full Name cytochrome c, testis
Background Cytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases
Function Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases (By similarity). [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30106 Mitochondria/Caspase Dependant Apoptosis Marker Antibody Duo (Caspase9, Cytochrome c)
ARG30110 Mitochondria/Caspase dependant Apoptosis Antibody Panel (Caspase3, Caspase9, Cytochrome c, PARP) (WB)
Related products:
Cytochrome C antibodies; Cytochrome C Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related poster download:
The Structure & Functions of Mitochondria.pdf
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody; Mitochondrial Marker antibody; Cytochrome-C fractionation Study antibody

Images (1) Click the Picture to Zoom In

  • ARG20006 anti-Cytochrome C antibody WB image

    Western blot: 20 µg of Mouse testis lysate stained with ARG20006 anti-Cytochrome C antibody at 2 µg/ml dilution.

Customer's Feedback

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Review for anti-Cytochrome C antibody

Application:WB

Sample:Mouse testis

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:2 µg/ml

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Probing apoptosis signaling proteins in single living cells for precision efficacy evaluation of anti-cancer drugs.

 / Human

Wen Yanrong et al.
Preprint.,  (2019)

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