anti-HMGB1 antibody [1F3]

anti-HMGB1 antibody [1F3] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat



Product Description

Mouse Monoclonal to Anti-High-mobility group protein B1, HMGB1

Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 1F3
Isotype IgG2b
Target Name HMGB1
Antigen Species Human
Immunogen Human full length protein expressed and purified form E. coli.
Conjugation Un-conjugated
Alternate Names HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 15289 Mouse HMGB1

GeneID: 3146 Human HMGB1

Swiss-port # P09429 Human High mobility group protein B1

Swiss-port # P63158 Mouse High mobility group protein B1

Gene Symbol HMGB1
Gene Full Name high mobility group box 1
Background HMGB1 is a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
Function HMGB1 is a DNA binding protein. It associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). [UniProt]
Highlight Related products:
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Related poster download:
HMGB1 Pathway.pdf
Calculated MW 25 kDa
PTM Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion (PubMed:17114460).
Acetylated on multiple sites upon stimulation with LPS (PubMed:22801494). Acetylation on lysine residues in the nuclear localization signals (NLS 1 and NLS 2) leads to cytoplasmic localization and subsequent secretion (By similarity). Acetylation on Lys-3 results in preferential binding to DNA ends and impairs DNA bending activity (By similarity).
Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB1 (HMGB1C23hC45hC106h), 2- disulfide HMGB1 (HMGB1C23-C45C106h) and 3- sulfonyl HMGB1 (HMGB1C23soC45soC106so).
Poly-ADP-ribosylated by PARP1 when secreted following stimulation with LPS (By similarity).
In vitro cleavage by CASP1 is liberating a HMG box 1-containing peptide which may mediate immunogenic activity; the peptide antagonizes apoptosis-induced immune tolerance (PubMed:24474694). Can be proteolytically cleaved by a thrombin:thrombomodulin complex; reduces binding to heparin and proinflammatory activities (By similarity).

Images (5) Click the Picture to Zoom In

  • ARG10756 anti-HMGB1 antibody [1F3] WB image

    Western blot: 20 µg of COLO205, HCT116, HT29, SW480 and SW620 cell lysates stained with ARG10756 anti-HMGB1 antibody [1F3] (1:2000) and ARG65680 anti-GAPDH antibody (1:10000).

  • ARG10756 anti-HMGB1 antibody [1F3] WB image

    Western blot: 30 µg of 293T and 3T3 cell lysates stained with ARG10756 anti-HMGB1 antibody [1F3] at 1:1000 dilution.

  • ARG10756 anti-HMGB1 antibody [1F3] ICC/IF image

    Immunocytochemistry: HeLa cells grown in tissue culture and stained with ARG10756 anti-HMGB1 antibody [1F3] (red) and co-stained with chicken polyclonal antibody to Vimentin (green); DNA (blue). ARG10756 anti-HMGB1 antibody [1F3] reveals strong nuclear staining which overlaps with the DNA stain.

  • ARG10756 anti-HMGB1 antibody [1F3] ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG10756 anti-HMGB1 antibody [1F3] (red) at 1:2000 dilution and costained with Rabbit pAb to GAPDH (green) at 1:2000 dilution.

    Clone 1F3 stains the chromatin binding protein HMGB1, which is localized in the nuclei. In contrast the GAPDH antibody produces strong cytoplasmic staining of cells.

  • ARG10756 anti-HMGB1 antibody [1F3] WB image

    Western blot: NIH/3T3, C6, HEK293, HeLa and SH-SY5Y cell lysates stained with ARG10756 anti-HMGB1 antibody [1F3] (green) at 1:2000 dilution.

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Sample:293T and 3T3

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC