anti-HMGB1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Gene Regulation antibody
Rabbit Polyclonal antibody recognizes HMGB1
|Tested Reactivity||Hu, Ms, Rat|
|Tested Application||ICC/IF, IHC-P, WB|
|Immunogen||Recombinant protein of Human HMGB1 (NP_002119.1)|
|Full Name||high mobility group box 1|
|Alternate Names||HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Positive Control||Mouse kidney and SW480|
|Calculated MW||25 kDa|
|Purification||Affinity purification with immunogen.|
|Buffer||PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol|
|Preservative||0.02% Sodium azide|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||high mobility group box 1|
|Function||DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells (By similarity). [UniProt]|
HMGB1 antibodies; HMGB1 ELISA Kits; Anti-Rabbit IgG secondary antibodies;
HMGB1 ELISA Kit for your research
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|Research Area||Gene Regulation antibody|
|PTM||Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion (PubMed:17114460).
Acetylated on multiple sites upon stimulation with LPS (PubMed:22801494). Acetylation on lysine residues in the nuclear localization signals (NLS 1 and NLS 2) leads to cytoplasmic localization and subsequent secretion (By similarity). Acetylation on Lys-3 results in preferential binding to DNA ends and impairs DNA bending activity (By similarity).
Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB1 (HMGB1C23hC45hC106h), 2- disulfide HMGB1 (HMGB1C23-C45C106h) and 3- sulfonyl HMGB1 (HMGB1C23soC45soC106so).
Poly-ADP-ribosylated by PARP1 when secreted following stimulation with LPS (By similarity).
In vitro cleavage by CASP1 is liberating a HMG box 1-containing peptide which may mediate immunogenic activity; the peptide antagonizes apoptosis-induced immune tolerance (PubMed:24474694). Can be proteolytically cleaved by a thrombin:thrombomodulin complex; reduces binding to heparin and proinflammatory activities (By similarity).
Images (3) Click the Picture to Zoom In
ARG55034 anti-HMGB1 antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG55034 anti-HMGB1 antibody.
ARG55034 anti-HMGB1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG55034 anti-HMGB1 antibody at 1:100 dilution.
ARG55034 anti-HMGB1 antibody WB image
Western blot: Mouse kidney and SW480 cell lysates stained with ARG55034 anti-HMGB1 antibody.