ARG55034

anti-HMGB1 antibody

anti-HMGB1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Gene Regulation antibody

Overview

Product Description

Rabbit Polyclonal antibody recognizes HMGB1

Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name HMGB1
Antigen Species Human
Immunogen Recombinant protein of Human HMGB1 (NP_002119.1)
Conjugation Un-conjugated
Full Name high mobility group box 1
Alternate Names HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-PAssay-dependent
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse kidney and SW480
Calculated MW 25 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 15289 Mouse HMGB1

GeneID: 3146 Human HMGB1

Swiss-port # P09429 Human High mobility group protein B1

Swiss-port # P63158 Mouse High mobility group protein B1

Gene Symbol HMGB1
Gene Full Name high mobility group box 1
Function DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells (By similarity). [UniProt]
Highlight Related products:
HMGB1 antibodies; HMGB1 ELISA Kits; Anti-Rabbit IgG secondary antibodies;
Related news:
HMGB1 ELISA Kit for your research
Related poster download:
HMGB1 Pathway.pdf
Research Area Gene Regulation antibody
PTM Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion (PubMed:17114460).
Acetylated on multiple sites upon stimulation with LPS (PubMed:22801494). Acetylation on lysine residues in the nuclear localization signals (NLS 1 and NLS 2) leads to cytoplasmic localization and subsequent secretion (By similarity). Acetylation on Lys-3 results in preferential binding to DNA ends and impairs DNA bending activity (By similarity).
Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB1 (HMGB1C23hC45hC106h), 2- disulfide HMGB1 (HMGB1C23-C45C106h) and 3- sulfonyl HMGB1 (HMGB1C23soC45soC106so).
Poly-ADP-ribosylated by PARP1 when secreted following stimulation with LPS (By similarity).
In vitro cleavage by CASP1 is liberating a HMG box 1-containing peptide which may mediate immunogenic activity; the peptide antagonizes apoptosis-induced immune tolerance (PubMed:24474694). Can be proteolytically cleaved by a thrombin:thrombomodulin complex; reduces binding to heparin and proinflammatory activities (By similarity).

Images (3) Click the Picture to Zoom In

  • ARG55034 anti-HMGB1 antibody ICC/IF image

    Immunofluorescence: A549 cells stained with ARG55034 anti-HMGB1 antibody.

  • ARG55034 anti-HMGB1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG55034 anti-HMGB1 antibody at 1:100 dilution.

  • ARG55034 anti-HMGB1 antibody WB image

    Western blot: Mouse kidney and SW480 cell lysates stained with ARG55034 anti-HMGB1 antibody.